FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496663
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21970.44235225897640856No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG19930.40127813024123No Hit
GGTGTTTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG19020.3829558473250474No Hit
GGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG16740.337049468150436No Hit
CAAAACACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG15020.3024183400011678No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14310.2881229324511792No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13330.26839124315682866No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12960.2609415237293698No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12110.2438273034230454No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11370.22892786456812766No Hit
CAACTATTGTGGTTTCATATTTCCTGTCTTACTTTTGGAAGAGAAACTGT11140.2242969578970046No Hit
CTATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATA10890.21926336368926214No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC10580.21302170687166147No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10300.2073840813589899No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT9980.2009410807730795No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9880.19892764308998254No Hit
CTATTAGAGATCTCCTCGACACCGCCTCTGCTTTGTATCGGGAGGCCTTA9810.19751823671181465No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9650.19429673641885947No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9210.18543761061323272No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9130.18382686046675512No Hit
ATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATATT8760.17637714103929625No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAACCACATTGTGTAAAA8740.17597445350267688No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8650.1741623595878896No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8620.17355832828296047No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8260.16630995262381132No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTAG8210.16530323378226283No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG7960.16026963957452037No Hit
GAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCA7700.1550347015984682No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC7220.1453702007196026No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7020.14134332535340866No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6900.13892720013369225No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6880.13852451259707285No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6820.13731644998721468No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6770.1363097311456662No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6700.13490032476749828No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6620.13328957462102067No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6540.1316788244745431No Hit
CAATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTA6460.13006807432806552No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC6390.1286586679498976No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6380.12845732418158792No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTAG6370.12825598041327824No Hit
GTCTAGCCTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCG6230.12543716765694243No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6180.12443044881539395No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6150.12382641751046485No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6110.12302104243722604No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6090.12261835490060666No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT6050.12181297982736786No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6040.12161163605905817No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTCAACTTCAT5960.12000088591258057No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCTTTATATGCATGTATA5950.11979954214427087No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5940.11959819837596115No Hit
CTTTAATCCTGAGTGGCAAATTCCCTCCTTTCCTAATATTCATTTACAGG5840.11758476069286417No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC5820.11718207315624478No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5740.11557132300976719No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5700.11476594793652839No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT5660.11396057286328959No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5660.11396057286328959No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5640.1135578853266702No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5580.11234982271681199No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAATTCCCTCCTTTCCTAAT5560.1119471351801926No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5560.1119471351801926No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5550.11174579141188291No Hit
CTCCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5520.11114176010695381No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5430.10932966619216651No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5430.10932966619216651No Hit
TCAAAACACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACG5310.10691354097245015No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA5300.10671219720414042No Hit
TCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGT5290.10651085343583074No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5260.10590682213090163No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5230.10530279082597255No Hit
CTCCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5180.10429607198442406No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5150.10369204067949495No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5090.10248397806963676No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5090.10248397806963676No Hit
CTCACTAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC5080.10228263430132704No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5050.10167860299639797No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5020.10107457169146886No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5020.10107457169146886No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5020.10107457169146886No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT5000.10067188415484947No Hit
TAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCCCAGTCC4980.10026919661823006No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4970.10006785284992037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)