FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496663
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21850.43993613375669216No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG17150.3453045626511337No Hit
GGTGTTTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG15150.3050358089891939No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15020.3024183400011678No Hit
GGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG13600.27382752490119056No Hit
CAAAACACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG13590.27362618113288084No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12380.2492635851674073No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11620.23396145877587013No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11480.23114264601953438No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC11400.22953189587305678No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10560.21261901933504207No Hit
CAACTATTGTGGTTTCATATTTCCTGTCTTACTTTTGGAAGAGAAACTGT10050.20235048715124743No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT10030.20194779961462805No Hit
CTATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATA9990.20114242454138923No Hit
CTATTAGAGATCTCCTCGACACCGCCTCTGCTTTGTATCGGGAGGCCTTA9790.19711554917519525No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9520.19167926743083338No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9310.1874510482963297No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9180.1848335793083036No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9170.18463223553999392No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9040.18201476655196783No Hit
ATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATATT8730.1757731097343672No Hit
GAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCA8360.1683233903069083No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8320.16751801523366952No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAACCACATTGTGTAAAA8210.16530323378226283No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTAG8150.16409517117240463No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7870.15845754565973308No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7540.15181320130551298No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7520.15141051376889358No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7330.14758498217100932No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7310.14718229463438992No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG7230.14557154448791235No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6940.13973257520693105No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6740.13570569984073708No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6540.1316788244745431No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6280.12644388649849092No Hit
CTTTAATCCTGAGTGGCAAATTCCCTCCTTTCCTAATATTCATTTACAGG6230.12543716765694243No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6220.12523582388863272No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCTTTATATGCATGTATA6170.12422910504708423No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6170.12422910504708423No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTAG6150.12382641751046485No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTCAACTTCAT6070.12221566736398724No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6070.12221566736398724No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6050.12181297982736786No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6040.12161163605905817No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6020.12120894852243876No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6010.12100760475412906No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5950.11979954214427087No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5940.11959819837596115No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT5910.11899416707103208No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5870.11818879199779328No Hit
GTCTAGCCTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCG5830.11738341692455448No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT5830.11738341692455448No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5830.11738341692455448No Hit
CTCCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5820.11718207315624478No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5770.11617535431469629No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5700.11476594793652839No Hit
CAATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTA5650.1137592290949799No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC5640.1135578853266702No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGTCCGTGTG5620.11315519779005079No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5600.1127525102534314No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5410.10892697865554712No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5400.10872563488723741No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5280.10630950966752103No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5260.10590682213090163No Hit
CTCACTAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC5220.10510144705766285No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5210.10490010328935313No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5200.10469875952104345No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5140.10349069691118526No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5050.10167860299639797No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5040.10147725922808827No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC4980.10026919661823006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)