FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508063
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31490.6198050241800722No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG20040.3944392722949713No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT15300.3011437557940649No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13890.27339129202480794No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11290.22221653613823483No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT10500.20666728338808374No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT10390.20450219756211335No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTACCAATTACATATCCCATGAA9080.1787179936346477No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8940.17596242985613988No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8780.17281321410927386No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8470.1667116085997209No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8360.16454652277375048No Hit
CTTTACCACAGGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAA8290.1631687408844966No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8180.1610036550585262No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7800.15352426765971935No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7640.15037505191285333No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7470.14702901018180817No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7370.1450607503400169No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6970.1371877109728518No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6940.1365972330203144No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6940.1365972330203144No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6800.13384166924180663No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6680.1314797574316571No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6560.12911784562150758No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6480.12754323774807455No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6440.12675593381135805No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6430.1265591078271789No Hit
GTGTAAACGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6420.12636228184299977No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6370.12537815192210414No Hit
CCCTTAACTTCATGGGATATGTAATTGGTAGTTGGGGTACTTTACCACAG6320.12439402200120851No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6290.12380354404867112No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6270.12340989208031287No Hit
CTAACAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA6220.12242576215941725No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6110.12026067633344682No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA6070.11947337239673034No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5920.11652098263404342No Hit
CGTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5910.11632415664986429No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA5840.11494637476061041No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5820.11455272279225215No Hit
GTTTGGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5820.11455272279225215No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5580.1098288991719531No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5480.1078606393301618No Hit
ATTTACATACTCTGTGGAAAGCTGGCATTCTATATAAGAGAGAAACTACA5430.10687650940926618No Hit
CTACTGTACCTGTCTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5350.10530190153583315No Hit
CTTTATATGCGTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT5320.10471142358329578No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5270.10372729366240013No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5260.10353046767822101No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5260.10353046767822101No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT5150.10136538185225061No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTACCAATTACATATCCC5120.10077490389971322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)