FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706938
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40120.5675179435820397No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG23110.3269027835538619No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT20130.2847491576347572No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17400.2461319097290003No Hit
GTACGGGACCATGCAAGACCTGCACCAGTCCTGCTCAAGGAAACTCTACG17270.2442929931620595No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA16260.23000602598813474No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT15070.21317286664459967No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC14040.19860298922960712No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC13940.19718843802426803No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCAAAG13200.18672075910475883No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC13140.1858720283815554No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC11410.16140029252918925No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10870.1537617160203582No Hit
GGATCATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA10440.14767914583740016No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA10430.14753769071686626No Hit
CCTAATATCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA10010.14159657565444211No Hit
CATGTATACAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCC9940.14060638981070475No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG9760.1380601976410944No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9640.1363627361946875No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9510.1345238196277467No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT9120.1290070699269243No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9020.1275925187215852No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8990.1271681533599835No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC8910.12603651239571223No Hit
GTGTTGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA8720.12334886510556797No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT8600.12165140365916105No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA8540.1208026729359576No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8330.11783211540474553No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT7940.11231536570392311No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7900.1117495452217875No Hit
ATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG7570.1070815262441685No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA7450.1053840647977616No Hit
CTATCAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG7410.10481824431562599No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7370.10425242383349034No Hit
GTATGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA7270.10283787262815128No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7240.10241350726654953No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7220.10213059702548172No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT7200.10184768678441392No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7170.10142332142281218No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTTCTTTTTTCA7110.10057459069960874No Hit
ATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA7090.10029168045854091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)