FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706938
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39940.5649717514124294No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG25860.3658029417006866No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT17760.25122429406822094No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16810.23778605761749969No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA15520.21953834706862554No Hit
GTACGGGACCATGCAAGACCTGCACCAGTCCTGCTCAAGGAAACTCTACG15330.2168506997784813No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCAAAG14680.20765611694377725No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT13710.19393497025198816No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC12830.1814869196450042No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC12420.175687259703114No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC11940.1688974139174864No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC10710.15149843409181568No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA10610.15008388288647662No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10600.1499424277659427No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10190.14414276782405247No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9850.1393332937258996No Hit
CCTAATATCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA9440.13353363378400934No Hit
GGATCATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA9430.13339217866347544No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9350.13226053769920418No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9240.13070453137333118No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8950.12660233287784783No Hit
GTGTTGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA8930.12631942263678003No Hit
CATGTATACAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCC8800.12448050606983921No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8750.12377323046716968No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8720.12334886510556797No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT8640.1222172241412967No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA8150.11528592323513519No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC8030.11358846178872829No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7710.10906189793164323No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7690.10877898769057542No Hit
ATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA7550.1067986160031007No Hit
GTATGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA7410.10481824431562599No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7270.10283787262815128No Hit
ATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG7080.10015022533800699No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT7080.10015022533800699No Hit
CTTTACCACAGGATCATATTATACAAAAAATCAAGCAATGTTTTCGAAAA7070.1000087702174731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)