FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457059
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21610.47280548025528435No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16590.36297283282902204No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14120.308931669653152No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11070.24220067868699663No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11060.2419818885526814No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10700.23410544371733188No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10610.23213633250849455No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10160.22229077646430767No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9720.2126640105544361No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9440.20653788679360874No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9370.20500635585340185No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8590.1879407253768113No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8240.1802830706757771No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8160.17853274960125498No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8140.17809516933262445No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8080.17678242852673287No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7900.17284420610905812No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7240.15840405724425075No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7010.1533718841549997No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6720.14702697025985703No Hit
GAATTCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAA6710.14680818012554178No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAGACAATA6390.13980689582745334No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6350.1389317352901923No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT6330.13849415502156176No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6160.1347747227382023No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6050.1323680312607344No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6020.1317116608577886No Hit
GGAACATATTGTCTTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6000.13127408058915807No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5930.12974254964895124No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5850.12799222857442913No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5840.12777343844011385No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5800.1268982779028528No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5610.1227412653508628No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5550.12142852454497123No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5470.11967820347044912No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5460.11945941333613384No Hit
GTCTTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5240.11464603038119806No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5240.11464603038119806No Hit
CATTTGAACCCCAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5230.11442724024688278No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5230.11442724024688278No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5220.11420845011256753No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAGACAAT5100.11158296850078436No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT5090.1113641783664691No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5050.11048901782920804No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAGACAATATGTT5050.11048901782920804No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4960.10851990662037068No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTCTTAAAA4960.10851990662037068No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4930.10786353621742488No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG4920.10764474608310963No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA4920.10764474608310963No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4800.10501926447132647No Hit
GTTTTAAGACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4780.10458168420269594No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAG4690.10261257299385856No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4660.10195620259091276No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4630.10129983218796698No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4610.10086225191933645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)