FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446251
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50551.1327705708222502No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11300.2532207210740144No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCAGG10530.23596585777959042No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9000.20168022032443625No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8180.18330491136154317No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8130.18218446569307406No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7320.1640332458638748No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7070.15843101752152935No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC7060.15820692838783554No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG7010.15708648271936645No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7010.15708648271936645No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6800.15238061091179628No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6440.14431340209881882No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6350.14229659989557447No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6330.14184842162818684No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT6260.1402797976923301No Hit
ATTTACAGGTAGTTTCCGAAAACATTCCTTAAGTTTTTGTACAATATGTT6060.13579801501845376No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6020.13490165848367847No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5960.13355712368151557No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5890.13198849974565885No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5890.13198849974565885No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5810.1301957866761083No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5810.1301957866761083No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5800.12997169754241447No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5760.1290753410076392No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5740.12862716274025157No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT5690.1275067170717825No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5680.12728262793808867No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5530.1239212909326814No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5480.12280084526421228No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5120.11473363645123484No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCTG5010.11226865598060284No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4920.11025185377735848No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTT4900.10980367550997083No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4860.10890731897519558No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4810.1077868733067265No Hit
GGAACATATTGTACAAAAACTTAAGGAATGTTTTCGGAAACTACCTGTAA4760.10666642763825739No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4750.10644233850456357No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4710.1055459819697883No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4650.1042014471676254No Hit
GGATTAAAGGTAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC4630.10375326890023776No Hit
CTACTGTACCTACCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4590.1028569123654625No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGACGTTCACGGTGGTCTCCATGC4470.10016784276113666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)