FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446251
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50601.1338910164907192No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCAGG12680.2841450215237613No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10130.2270022924318377No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT10010.22431322282751184No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8500.19047576363974533No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC8100.18151219829199264No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7990.17904721782136063No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG7640.171204098142077No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7210.16156826539324282No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6980.156414215318285No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6960.15596603705089737No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6860.1537251457139592No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6760.151484254377021No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6680.14969154130747045No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6550.14677838256945083No Hit
ATTTACAGGTAGTTTCCGAAAACATTCCTTAAGTTTTTGTACAATATGTT6420.1438652238314312No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6310.14140024336079918No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6290.14095206509341154No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT6290.14095206509341154No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6070.13602210415214755No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5990.13422939108259702No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCTG5890.13198849974565885No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5880.131764410611965No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5860.13131623234457737No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5800.12997169754241447No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5730.12840307360655775No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5420.12145631046204938No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5380.12055995392727412No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5360.12011177565988647No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTT5320.11921541912511122No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT5260.11787088432294829No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5190.11630226038709156No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5170.11585408211970394No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5150.1154059038523163No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5150.1154059038523163No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5040.1129409233816843No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4960.11114821031213376No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4840.10845914070780793No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4810.1077868733067265No Hit
GTCTATTTACAGGTAGTTTCCGAAAACATTCCTTAAGTTTTTGTACAATA4760.10666642763825739No Hit
GGATTAAAGGTAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC4690.10509780370240067No Hit
CTACTGTACCTACCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4670.10464962543501302No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA4530.10151237756329957No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA4510.10106419929591196No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4500.10084011016221812No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4500.10084011016221812No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4470.10016784276113666No Hit
GGAACATATTGTACAAAAACTTAAGGAATGTTTTCGGAAACTACCTGTAA4470.10016784276113666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)