FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393535
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCGGA25340.6439071493005705No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23490.5968973534755485No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA20240.5143125770261857No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG15340.38980014484099257No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14340.36438944439503473No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13970.3549874852300304No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG12310.3128057224897404No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11730.2980675162310849No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA11470.2914607341151359No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG10120.25715628851309286No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG10070.255885753490795No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9920.2520741484239013No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC9790.2487707573659268No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9160.2327620160849734No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9080.23072916004929678No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8940.22717166198686264No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8800.22361416392442857No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8800.22361416392442857No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8770.22285184291104984No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8640.2195484518530753No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8120.2063348876211773No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7920.20125274753198574No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7800.1982034634784708No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7580.19261310938036008No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7540.19159668136252175No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7530.19134257435806218No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7450.18930971832238555No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7390.1877850762956281No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7310.18575222025995147No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7220.18346525721981527No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGTAT6940.1763502610949471No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6790.1725386560280534No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6760.17177633501467468No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6720.17075990699683638No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6650.16898115796561933No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC6390.1623743758496703No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6390.1623743758496703No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6340.1611038408273724No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6320.16059562681845324No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6020.1529724166846659No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCTTGGCTCAGTTTACTAGTG6020.1529724166846659No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5990.15221009567128718No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5940.1509395606489893No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5930.15068545364452973No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5930.15068545364452973No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5900.14992313263115098No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5860.14890670461331265No Hit
CGGTGGCCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5860.14890670461331265No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTCTCCTCTGCCGAT5810.14763616959101478No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5770.14661974157317645No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5710.145095099546419No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5640.14331635051520195No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5610.14255402950182322No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5580.1417917084884445No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5520.14026706646168702No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5430.13798010342155081No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5420.13772599641709124No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5390.13696367540371251No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5350.1359472473858742No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5350.1359472473858742No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5330.13543903337695504No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA5310.1349308193680359No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT5290.13442260535911674No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5290.13442260535911674No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTCAGTAAACAGTATC5270.1339143913501976No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5260.133660284345738No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5250.1334061773412784No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5180.13162742831006136No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5170.13137332130560178No Hit
GTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA5170.13137332130560178No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5160.1311192143011422No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5150.13086510729668263No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA5110.1298486792788443No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATGTGTT5110.1298486792788443No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5110.1298486792788443No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5030.1278158232431677No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4990.12679939522532938No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4970.12629118121641023No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4940.1255288602030315No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4930.12527475319857193No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4910.12476653918965276No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4900.12451243218519319No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4900.12451243218519319No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4860.12349600416735487No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTACCACAG4860.12349600416735487No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTG4820.12247957614951656No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4810.12222546914505698No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4800.12197136214059741No Hit
CAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4780.12146314813167826No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTGGCCTC4770.12120904112721867No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4760.1209549341227591No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4760.1209549341227591No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4740.12044672011383994No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCAC4730.12019261310938037No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4730.12019261310938037No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4730.12019261310938037No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4720.11993850610492078No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4620.11739743606032502No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4590.11663511504694628No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4590.11663511504694628No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4580.1163810080424867No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4580.1163810080424867No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4550.11561868702910796No Hit
ATGTGTTCCTGTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAA4540.11536458002464839No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4530.11511047302018881No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4450.11307761698451217No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4420.11231529597113343No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA4400.11180708196221428No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4400.11180708196221428No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4380.11129886795329513No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4370.11104476094883554No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4370.11104476094883554No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4360.11079065394437597No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4310.10952011892207808No Hit
GCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGG4290.10901190491315893No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4240.10774136989086104No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG4240.10774136989086104No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4230.10748726288640147No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4210.1069790488774823No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4200.10672494187302273No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4190.10647083486856315No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4190.10647083486856315No Hit
CATTACCACAGGAACACATTGTACAAAAAATCAAAATGTGTTTTAGGAAA4130.10494619284180569No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4120.10469208583734611No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4120.10469208583734611No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4090.10392976482396737No Hit
GGTTGGGGCTTGGTCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4080.1036756578195078No Hit
GCCTATGACCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC4070.10342155081504822No Hit
CTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4060.10316744381058865No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4060.10316744381058865No Hit
GCATATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCGGAAACTAC4050.10291333680612906No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC4020.10215101579275034No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG4020.10215101579275034No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4010.10189690878829076No Hit
GTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4000.10164280178383117No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3970.10088048077045245No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC3960.10062637376599287No Hit
CCTGCATATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCGGAAAC3960.10062637376599287No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC3940.10011815975707371No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTA3940.10011815975707371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)