FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465796
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG18980.40747451674123436No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA17330.3720512842531924No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15230.3269671701775026No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT13600.2919733102044672No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11950.25655007771642524No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11780.25290041133886937No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT11640.2498948037338234No Hit
ATCCTAACCTTACCAAATATCTGCCCTTGGACAAAGGCATTAAACCATAT11560.24817731367379714No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11430.24538639232625442No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10660.2288555504985015No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA9960.21382751247327159No Hit
GTATAGGCCTCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG9510.20416663088562376No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG9310.19987290573555808No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9150.19643792561550552No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT8840.1897826516329037No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG8520.1829126913927986No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT8290.17797490747022301No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT8180.1756133586376869No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG8080.17346649606265405No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8030.17239306477513763No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7900.16960214342759491No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7770.16681122208005222No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7680.16487904576252266No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCA7590.1629468694449931No Hit
ATTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA7550.16208812441497997No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7470.1603706343549537No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7400.1588678305524307No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7200.154574105402365No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6920.14856289019227303No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC6910.14834820393476975No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC6840.14684540013224673No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6730.14448385129971061No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6670.1431957337546909No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6590.14147824369466463No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6500.13954606737713507No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6490.13933138111963178No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6390.13718451854459893No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6360.13654045977208906No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6350.1363257735145858No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6310.13546702848457265No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6240.13396422468204966No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6180.13267610713702996No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAA6140.1318173621070168No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6050.12988518578948724No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6000.12881175450197083No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5930.12730895069944784No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5890.12645020566943468No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG5880.1262355194119314No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5860.12580614689692485No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5840.12537677438191827No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5830.12516208812441498No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5730.12301522554938214No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5730.12301522554938214No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5700.12237116677687229No Hit
GTTTTATTAGGGTTCAAATGTATACCCAGAGACAAAAGAAAATTGGTAAT5680.12194179426186572No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5670.12172710800436243No Hit
ATGTAATTGGATGTTGGGGCACTTTACCGCAAGAACATATTGTACTAAAA5570.11958024542932959No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC5550.11915087291432301No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5530.11872150039931645No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5520.11850681414181316No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5510.11829212788430987No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5500.11807744162680658No Hit
GGATAGAACCTAGCAGGCATAATTAATTTCAATCTCCTTTTTTCATTAAC5470.11743338285429673No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5360.1150718340217606No Hit
ATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGG5290.11356903021923762No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTA5290.11356903021923762No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT5280.11335434396173433No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAGATATTTGGTAAGGTT5270.11313965770423104No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGACCAGTAAAGTTTCCCAC5240.11249559893172119No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5240.11249559893172119No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT5220.11206622641671463No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5210.11185154015921134No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5200.11163685390170805No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC5190.11142216764420476No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT5160.11077810887169491No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5150.11056342261419162No Hit
CTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG5070.10884593255416534No Hit
CTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT5060.10863124629666207No Hit
TTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACAC5050.10841656003915878No Hit
GTTCTATCCTAACCTTACCAAATATCTGCCCTTGGACAAAGGCATTAAAC4930.10584032494911937No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC4920.10562563869161608No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4790.10283471734407337No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAGATATTTGGTA4750.10197597231406023No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4730.10154659979905367No Hit
GTACAGTAGAAGAATAAAGACCAGTAAAGTTTCCCACCTTATGAGTCCAA4720.10133191354155038No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT4660.10004379599653068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)