FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423583
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22940.5415703651940705No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14700.34703942320631376No Hit
CTACTGTACCTGTCTTTAATTCTGAATGGCAAACTCCCTCCTTTCCTCAC14560.3437342858424441No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13880.3276807615036486No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13850.32697251778281944No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13780.3253199491008846No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13780.3253199491008846No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12550.2962819565468869No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG12120.2861304632150015No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11890.2807005946886443No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCTTAT11130.26275842042763753No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10910.2575646331415567No Hit
GTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10890.2570924706610039No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAACAAG10700.25260692709575217No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10640.25119043965409377No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCGTTGACATACT10040.23702556523750953No Hit
CATTAACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAG9740.22994312802921743No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9580.22616582818479497No Hit
CATGTATACAATCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCC9540.22522150322368933No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG9420.2223885283403725No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9340.22049987841816127No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9080.21436176617097477No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9030.21318135996959273No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG8830.20845973516406463No Hit
ATTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACA8690.205154597800195No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8100.19122580462388716No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGGGC8040.18980931718222877No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7930.1872124235391883No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7820.18461552989614785No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG7630.18012998633089616No Hit
ATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTCTGCCAAGTG7560.17847741764896136No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7550.17824133640868495No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA7360.1737557928434333No Hit
GGTTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7350.17351971160315688No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7290.17210322416149845No Hit
GGGTAAAGGTTCATATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA6980.16478470571292994No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6960.16431254323237712No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC6960.16431254323237712No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6780.16006308090740184No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6650.1569940247838086No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6650.1569940247838086No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6610.15604969982270298No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6580.15534145610187378No Hit
TTTACATACTCTGTGGAAGGCTGGCATCCTATATAAGAGAGAAACTACAC6470.15274456245883333No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6320.14920334385468725No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6310.14896726261441087No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAATTTCAT6090.14377347532832999No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6050.14282915036722435No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATACGCATGTATA6030.14235698788667156No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6020.14212090664639515No Hit
GGTTCATATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGAGAAAGTG6010.14188482540611877No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6010.14188482540611877No Hit
CCTTTATACGCATGTATACAATCTAAGCAAGCTTTCACTTTCTCGCCAAC5910.1395240130033547No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5800.13692711936031426No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5750.13574671315893225No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5610.13244157579506258No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5600.1322054945547862No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5590.1319694133145098No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5500.12984468215202216No Hit
CAATATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTCTGCCA5490.12960860091174575No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5420.12795603222981095No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC5410.12771995098953454No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5370.1267756260284289No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5330.1258313010673233No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5280.12465089486594126No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5250.12394265114511205No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5240.12370656990483567No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5210.12299832618400644No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5140.12134575750207162No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT5140.12134575750207162No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5120.1208735950215188No Hit
GTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5110.1206375137812424No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5090.12016535130068959No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5010.11827670137847836No Hit
AATTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA4980.11756845765764916No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4970.11733237641737275No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4960.11709629517709635No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4940.11662413269654354No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4920.11615197021599072No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4920.11615197021599072No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4910.11591588897571431No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4900.11567980773543791No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC4890.11544372649516152No Hit
GTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA4890.11544372649516152No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4880.11520764525488512No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4840.11426332029377949No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4810.11355507657295028No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4800.1133189953326739No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4790.11308291409239749No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4760.11237467037156826No Hit
CATATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG4720.11143034541046264No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4700.11095818292990985No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4600.1085973705271458No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4560.10765304556604018No Hit
GGATAATAAGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4550.10741696432576377No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4540.10718088308548739No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC4540.10718088308548739No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4530.10694480184521098No Hit
ATCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTA4480.10576439564382895No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4470.10552831440355254No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4460.10529223316327614No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4440.10482007068272335No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4400.10387574572161772No Hit
GATTGTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4390.10363966448134132No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT4340.1024592582799593No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4340.1024592582799593No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCATTCAGAATTAAAG4330.1022231770396829No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4330.1022231770396829No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4320.10198709579940649No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGG4290.10127885207857727No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4270.10080668959802448No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4260.10057060835774807No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4260.10057060835774807No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC4240.10009844587719527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)