FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397681
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20080.5049273161151777No Hit
GTCCAGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC17190.4322560041842582No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAAAGTCCAA16180.406858763682449No Hit
GTATAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG13970.3512865839705694No Hit
ATATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT13900.34952637918331525No Hit
CTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11160.28062693465365457No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10440.26252197112761233No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10240.2574928145926006No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG10230.25724135676585No Hit
CATTAAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGATTGAGACCT10130.25472677849834413No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10100.2539724050180924No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9890.2486917906563301No Hit
GGGCTATACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG9170.23058682713028786No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT9160.23033536930353726No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9040.22731787538253023No Hit
TCCTCGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCAGTGCTGGTTGTTG8740.21977414058001263No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA8550.2149964418717515No Hit
GCACTGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8330.2094643696832386No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA8100.2036808396679751No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8060.20267500836097274No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7900.19865168313296333No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTTGTAACATT7710.19387398442470222No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7610.19135940615719635No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7550.18985065919669283No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7460.18758753875593753No Hit
GCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGA7420.1865817074489352No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7390.18582733396868342No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7180.18054671960692112No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7000.17602047872541057No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6950.17476318959165763No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6880.17300298480440351No Hit
GCACTGGACCATGCAAAACCTGCACGACTCCTGCTCGAGGAACCTCTATG6870.17275152697765295No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAGAGAGAGTCCACACGTAGCGCC6830.1717456956706506No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6580.16545925000188594No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCA6550.16470487652163418No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6380.1604300934668742No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTTTGGAAGTAATGATTAACTACA6380.1604300934668742No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6360.15992717781337304No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6290.15816697302611893No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6230.15665822606561539No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6210.15615531041211422No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCGACAATGTCCTCCTGC6120.15389218997135895No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTAC6080.1528863586643566No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5970.15012032257010016No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5970.15012032257010016No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5960.14986886474334957No Hit
CATACAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCA5860.1473542864758437No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5800.14584553951534018No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA5620.14131929863382964No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5600.14081638298032845No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5540.13930763601982493No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5520.13880472036632377No Hit
GGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT5510.13855326253957317No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAAACGCGACATTATT5470.13754743123257082No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5420.1362901420988179No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5390.13553576861856614No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5220.13126098556380617No Hit
GTGTTGGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5220.13126098556380617No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5210.13100952773705557No Hit
CATTGAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGATTGAGACCT5160.12975223860330265No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5070.12748911816254738No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5030.12648328685554502No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5020.12623182902879443No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5020.12623182902879443No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4970.12497453989504151No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4910.12346579293453798No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4880.12271141945428621No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG4810.12095121466703211No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4770.11994538336002977No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4750.1194424677065286No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC4750.1194424677065286No Hit
GTATGTAGTTAATCATTACTTCCAAACGCGACATTATTTACACACTCTTT4680.11768226291927449No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4670.1174308050925239No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4640.11667643161227216No Hit
TGTACACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAA4550.11441331117151687No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4530.1139103955180157No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4520.11365893769126512No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4480.11265310638426276No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4440.11164727507726041No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4440.11164727507726041No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4430.11139581725050983No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4400.11064144377025809No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4400.11064144377025809No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4370.10988707029000631No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4340.10913269680975456No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4320.10862978115625337No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4280.10762394984925104No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4270.10737249202250046No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4260.10712103419574985No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4200.10561228723524634No Hit
GGGTAAAGGTTCAGATACTGTTTGCTTAAAAAGGCCTTGTAAGTTGCCGA4180.10510937158174516No Hit
GTGTAAATAATGTCGCGTTTGGAAGTAATGATTAACTACATACTCTGGAT4160.104606455928244No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4160.104606455928244No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG4120.10360062462124164No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4090.1028462511409899No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTGCTT4030.10133750418048637No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4020.10108604635373578No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC3990.10033167287348402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)