FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295167
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13690.46380523568014037No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11610.39333665348768665No Hit
CTCCTGAACATTGTTCACCTCATCATACAGCACTCAGGCAAGCTATTCTG10050.3404852168433463No Hit
CCAACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9640.3265947751611799No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7340.24867278523683203No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG7050.23884783868115336No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6910.23410476103358438No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6600.22360223195682447No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6480.2195367368303367No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6410.21716519800655223No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6250.21174453783790195No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT6210.21038937279573935No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6140.20801783397195486No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6060.20530750388762972No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT5950.20158080002168266No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5720.19378860102924783No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5540.18769035833951628No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5470.1853188195157318No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5280.17888178556545956No Hit
CTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA5220.17684903800221569No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5150.1744774991784312No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5040.17075079531248413No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4920.1666853001859964No Hit
GTATACACGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA4900.1660077176649151No Hit
CCACCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4880.16533013514383382No Hit
ATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAGACAGGTACAGT4820.16329738758058995No Hit
GTTCCGGAGACTCTAAGGCCTCCCGATATAGAGCTGAGGCGGTGTCGAGG4750.16092584875680546No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG4660.1578767274119397No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4610.15618277110923648No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4500.1524560672432894No Hit
CCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCAGC4390.14872936337734233No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4250.14398628572977332No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4180.14161474690598883No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4150.14059837312436688No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG4140.14025958186382623No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4110.1392432080822043No Hit
CTCCACCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4020.13619408673733852No Hit
ACACGTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA3990.1351777129557166No Hit
CTCCGGAACATTGTTCACCTCATCATACAGCACTCAGGCAAGCTATTCTG3990.1351777129557166No Hit
ACTATGGACTGTTTATCATGGCGCTGGCTCCAAGACCTTAGCCGGTCCGA3980.13483892169517595No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3860.13077342656868823No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3840.13009584404760696No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3820.12941826152652566No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3730.12636914018165987No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA3690.1250139751394973No Hit
CATTTGAACCCTAGTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3680.12467518387895667No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3620.12264243631571278No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3620.12264243631571278No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3610.12230364505517216No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3610.12230364505517216No Hit
CTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG3610.12230364505517216No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT3570.12094848001300959No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3530.11959331497084702No Hit
GCTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG3500.1185769411892251No Hit
GACTACTAATTCCCGGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGG3490.11823814992868445No Hit
GTGTATACACGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAA3430.11620540236544058No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3390.114850237323278No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3380.11451144606273737No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC3380.11451144606273737No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3350.11349507228111543No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3320.1124786984994935No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3310.11213990723895285No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCGTGTATA3300.11180111597841223No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3250.110107159675709No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3250.110107159675709No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3250.110107159675709No Hit
CTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA3230.10942957715462773No Hit
ACCAAGTAAAGATCACTGGCGCCACACGTGCATGGCGTCAAAGACCTGGC3230.10942957715462773No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3200.10841320337300578No Hit
GCGAACCCACACTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTC3190.10807441211246516No Hit
CGTGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3180.10773562085192451No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3170.10739682959138386No Hit
ACATAGAGGTTCCTTGAGCAGGAATTGTGCAGGTCTTGCATGGTCCCGTG3170.10739682959138386No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3130.10604166454922129No Hit
GCTTAGAGTGTATACACGCATATAAAGGCATTAAGGCAGGATAGCCACAT3110.10536408202814002No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3100.10502529076759937No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3010.10197616942273356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)