FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349770
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22360.6392772393287017No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16180.46258970180404263No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT15780.4511536152328673No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14250.4074105840981216No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC13080.37396003087743374No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT11360.3247848586213798No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCCGG11180.31963861966435086No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9350.26731852360122366No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9320.26646081710838554No Hit
CCCTTATAGGCACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCC9050.25874145867284215No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9040.2584555565085628No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8440.24130142665179977No Hit
CTATAAGGGTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGT8040.2298653400806244No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATTTCCTTTTTTCG7700.22014466649512535No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7100.20299053663836233No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6880.1967006890242159No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6870.19641478685993655No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT6600.18869542842439316No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC6290.17983246133173228No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6220.1778311461817766No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6190.17697343968893844No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6110.17468622237470338No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6080.17382851588186524No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6050.17297080938902706No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC5930.16953998341767448No Hit
CTCTTACAGTTAACGAAAAAAGGAAATTAAAATTGATCATGCCTGCTAGG5910.1689681790891157No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5900.16868227692483634No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5860.1675386682677188No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5810.16610915744632188No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATTTCCTTTTTTCG5790.1655373531177631No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5720.1635360379678074No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5710.16325013580352804No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5550.1586757011750579No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5490.1569602881893816No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5400.15438716871086713No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5260.15038453841095578No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5190.1483832232610001No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5190.1483832232610001No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5180.1480973210967207No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5170.14781141893244132No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5100.14581010378248563No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5050.1443805929610887No Hit
GTATAGGCCCCTTACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5000.1429510821396918No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4940.1412356691540155No Hit
GTTTCCGAAAACATTTTTTGATTTTTTGTACAATGTGTTCCTGTGGCAAG4930.1409497669897361No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4900.14009206049689796No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC4890.13980615833261859No Hit
ATTGACAACAGTACCAGCAGCCCCTCCTCCTGCCTCCACCAATCGGCAGT4880.13952025616833919No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT4850.13866254967550104No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4830.1380907453469423No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4830.1380907453469423No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4820.1378048431826629No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4810.1375189410183835No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC4800.13723303885410412No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4690.13408811504703091No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4650.13294450638991337No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4650.13294450638991337No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA4650.13294450638991337No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4630.1323727020613546No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC4630.1323727020613546No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4560.13037138691139893No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4560.13037138691139893No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4550.13008548474711953No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4540.12979958258284013No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATTTCCTTTTTTCGTTAAC4460.12751236526860507No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4430.12665465877576693No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4400.12579695228292878No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4390.1255110501186494No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4290.12265202847585556No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG4260.1217943219830174No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4230.12093661549017927No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4230.12093661549017927No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG4220.12065071332589988No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4190.11979300683306172No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4180.11950710466878234No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC4150.1186493981759442No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4090.1169339851902679No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4070.11636218086170912No Hit
ACCCTTATAGGCACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTC4050.11579037653315036No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4050.11579037653315036No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA4010.11464676787603283No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC4000.11436086571175344No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4000.11436086571175344No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA3980.11378906138319468No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT3940.11264545272607714No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3930.11235955056179776No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC3860.11035823541184206No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA3860.11035823541184206No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC3850.11007233324756267No Hit
GTGCTGGAGTTCCACTGCATGGCCTGCGGATGACTGTCTCTTAGAGGTGG3840.1097864310832833No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT3820.10921462675472454No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT3820.10921462675472454No Hit
TAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCATTCA3790.1083569202618864No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3740.10692740944048945No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAA3740.10692740944048945No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA3720.10635560511193069No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3710.10606970294765132No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3690.10549789861909253No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3690.10549789861909253No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3690.10549789861909253No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3670.10492609429053378No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3630.10378248563341623No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC3630.10378248563341623No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3630.10378248563341623No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3620.10349658346913687No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC3580.10235297481201933No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT3580.10235297481201933No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3560.10178117048346055No Hit
ATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCATTC3530.10092346399062241No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3520.10063756182634304No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3510.10035165966206365No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT3500.10006575749778425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)