FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576787
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT20080.3481354468807376No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19940.34570820770925836No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15660.27150403875260715No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA15350.26612943773004594No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA15250.2643956954647036No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA14080.2441109109601985No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14010.24289729137445884No Hit
GGGACTTGGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA13400.23232146355587074No Hit
GACCAATAACCTAACATCCTCCCCCAACTCCTCCCACTCAGTAAACACAC13370.23180134087626802No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12780.22157226151074835No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12660.21949177079233756No Hit
CATTAAGATTCCCGATGTTGAGATCTTCTGCGACGCGGTGATTGAGACCT12340.2139437955432421No Hit
GTTGTGAGTAGGCCCTGAGTCTGAGGGCTTCACCCCAACAGTCCCCCGTG12110.20995618833295482No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGG12020.2083958202941467No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA11690.20267447081851708No Hit
ATCTGCACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG11580.2007673543266405No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG11580.2007673543266405No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT11580.2007673543266405No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT11480.1990336120612982No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11110.1926187656795316No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10930.18949802960191545No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10610.18395005435282No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC10560.18308318322014885No Hit
ACCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG10480.181696189407875No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA10420.1806559440486696No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT10310.17874882755679308No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10300.17857545333025884No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA10210.17701508529145074No Hit
GACCTTTAACCTAACATCCTCCCCCAACTCCTCCCACTCAGTAAACACAC10190.1766683368383823No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10100.1751079687995742No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC9740.16886649664434183No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9450.16383864407484913No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9420.16331852139524644No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT9280.16089128222376717No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG9240.16019778531763024No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9230.16002441109109602No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAA9070.15725042346654833No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATTTGGTTTTGGGGGC9070.15725042346654833No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG9070.15725042346654833No Hit
CTCCAAAAGACACCAAGTATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8750.15170244821745288No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8730.15135569976438443No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG8720.15118232553785022No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8720.15118232553785022No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8710.15100895131131598No Hit
ATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGAC8700.15083557708478174No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8550.14823496368676825No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8530.1478882152336998No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8500.14736809255409708No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT8420.14598109874182325No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8390.14546097606222053No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCTAAGCGGCCCCGGGAGG8240.14286036266420707No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA8210.14234023998460438No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8180.14182011730500169No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7880.13661889050897472No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG7840.13592539360283779No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7830.13575201937630357No Hit
CTATTCTGTGTTGGGGTGAATTAATGAATCTAGCCACCTGGGTGGGAAGT7740.13419165133749547No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT7710.13367152865789278No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7710.13367152865789278No Hit
TTGTTCACCTCACCATACGGCAATCAGGCAAGCTATTGTGTGTTGGGGTG7660.1328046575252216No Hit
GTACAGACTTGGCCCCCAAAACCAAATCATCCATATAACTGAAAGCCAGA7600.13176441216601623No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT7570.13124428948641353No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA7540.13072416680681084No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7530.1305507925802766No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA7490.12985729567413967No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG7480.12968392144760546No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT7460.12933717299453698No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7410.1284703018618658No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTGTTCG7410.1284703018618658No Hit
ATTCTGTGTTGGGGTGAATTAATGAATCTAGCCACCTGGGTGGGAAGTAA7290.12638981114345504No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC7290.12638981114345504No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7270.12604306269038656No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7230.12534956578424966No Hit
CCTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC7220.12517619155771542No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7150.12396257197197579No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG7090.1229223266127704No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7060.1224022039331677No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6970.12084183589435961No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6930.12014833898822269No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6910.11980159053515421No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG6850.11876134517594883No Hit
CATTAAGATTCCCGAGGTTGAGATCTTCTGCGACGCGGTGATTGAGACCT6820.11824122249634614No Hit
AAGTCAACAATATCTGCACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC6790.11772109981674345No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6740.11685422868407229No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6730.11668085445753805No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAAGAA6720.11650748023100382No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6710.11633410600446958No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6650.1152938606452642No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTTTGTCAACGAATTGTGGGTCT6640.11512048641872998No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6630.11494711219219574No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6620.1147737379656615No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6590.1142536152860588No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTC6530.11321336992685341No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGCATAA6500.11269324724725073No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6490.11251987302071649No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6440.11165300188804533No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT6390.11078613075537418No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6390.11078613075537418No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6370.1104393823023057No Hit
ATTGTGTGTTGGGGTGAATTAATGAATCTAGCCACCTGGGTGGGAAGTAA6320.10957251116963454No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6300.10922576271656609No Hit
ATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTCCATGC6250.10835889158389493No Hit
TCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT6240.10818551735736069No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6210.10766539467775799No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6200.10749202045122377No Hit
CCCCACGGGGGACTGTTGGGGTGAAGCCCTCAGACTCAGGGCCTACTCAC6050.10489140705321029No Hit
CCAATAACCTAACATCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG6030.10454465860014182No Hit
CCTGATGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC6000.10402453592053913No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5990.10385116169400489No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5980.10367778746747065No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC5980.10367778746747065No Hit
CCTCAGACTCAGGGCCTACTCACAACTGTGCCAGCAGCTCCTCCTCCTGC5890.10211741942866258No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG5880.10194404520212834No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5870.1017706709755941No Hit
GCTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG5860.10159729674905989No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5860.10159729674905989No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5850.10142392252252565No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5820.10090379984292294No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT5820.10090379984292294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)