FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576787
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT19790.3431075943112449No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17790.3084327490043985No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA15530.2692501738076621No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA15140.26248857897282707No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA14420.25000563466236236No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14040.24341741405406156No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG13670.237002567672295No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA13230.22937410170478875No Hit
CATTAAGATTCCCGATGTTGAGATCTTCTGCGACGCGGTGATTGAGACCT12750.22105213883114563No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12400.21498404090244755No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC12170.2109964336921602No Hit
GGGACTTGGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA12080.2094360656533521No Hit
ATCTGCACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG11910.2064887038022702No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11900.20631532957573595No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGG11720.20319459349811977No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA11300.19591287598368204No Hit
GTTGTGAGTAGGCCCTGAGTCTGAGGGCTTCACCCCAACAGTCCCCCGTG11270.19539275330407932No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11120.19279213990606583No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10990.19053827496112083No Hit
GACCAATAACCTAACATCCTCCCCCAACTCCTCCCACTCAGTAAACACAC10890.18880453269577852No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC10470.18152281518134078No Hit
ACCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG10430.18082931827520385No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10310.17874882755679308No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10240.17753520797105343No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10140.17580146570571112No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT9890.17146711004235532No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG9870.17112036158928684No Hit
CTCCAAAAGACACCAAGTATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9810.17008011623008149No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAA9780.16955999355047877No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT9550.16557238634019145No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCTAAGCGGCCCCGGGAGG9520.16505226366058875No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9450.16383864407484913No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT9270.16071790799723296No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9210.15967766263802757No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG9180.15915753995842485No Hit
ATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGAC9170.15898416573189064No Hit
GACCTTTAACCTAACATCCTCCCCCAACTCCTCCCACTCAGTAAACACAC9090.15759717191961678No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT9000.15603680388080868No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC9000.15603680388080868No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATTTGGTTTTGGGGGC8960.15534330697467177No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG8870.15378293893586367No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8490.14719471832756287No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8320.14424735647648093No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8140.14112662039886473No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT8120.14077987194579628No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8100.14043312349272782No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG7990.13852600700085127No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7950.13783251009471434No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG7820.13557864514976933No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7800.13523189669670085No Hit
GTACAGACTTGGCCCCCAAAACCAAATCATCCATATAACTGAAAGCCAGA7760.13453839979056392No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7750.13436502556402968No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7680.1331514059782901No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7670.13297803175175585No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7650.13263128329868737No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7600.13176441216601623No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA7580.13141766371294777No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT7560.1310709152598793No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7550.13089754103334506No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7510.13020404412720812No Hit
CTATTCTGTGTTGGGGTGAATTAATGAATCTAGCCACCTGGGTGGGAAGT7370.12777680495572888No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7350.12743005650266043No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7280.1262164369169208No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7280.1262164369169208No Hit
CCTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC7130.12361582351890733No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT7110.12326907506583885No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6990.12118858434742809No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6980.12101521012089385No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6960.12066846166782538No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6940.12032171321475693No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6910.11980159053515421No Hit
ATTCTGTGTTGGGGTGAATTAATGAATCTAGCCACCTGGGTGGGAAGTAA6870.1191080936290173No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6850.11876134517594883No Hit
TTGTTCACCTCACCATACGGCAATCAGGCAAGCTATTGTGTGTTGGGGTG6840.1185879709494146No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT6720.11650748023100382No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA6690.11598735755140113No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGCATAA6640.11512048641872998No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6610.11460036373912726No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6580.11408024105952458No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6560.1137334926064561No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6490.11251987302071649No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTTTGTCAACGAATTGTGGGTCT6460.11199975034111379No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6440.11165300188804533No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG6430.1114796276615111No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6360.11026600807577146No Hit
CATTAAGATTCCCGAGGTTGAGATCTTCTGCGACGCGGTGATTGAGACCT6300.10922576271656609No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTGTTCG6280.10887901426349762No Hit
CCCCACGGGGGACTGTTGGGGTGAAGCCCTCAGACTCAGGGCCTACTCAC6260.10853226581042914No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC6260.10853226581042914No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACGGGTTGGGGCTTGGCCATAG6220.10783876890429223No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6190.10731864622468953No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6090.10558490395934722No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6090.10558490395934722No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6080.10541152973281298No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT6070.10523815550627874No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6020.10437128437360758No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC6010.10419791014707334No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6010.10419791014707334No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACACG6000.10402453592053913No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5960.10333103901440219No Hit
CCTCAGACTCAGGGCCTACTCACAACTGTGCCAGCAGCTCCTCCTCCTGC5890.10211741942866258No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5870.1017706709755941No Hit
TCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT5850.10142392252252565No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5840.10125054829599142No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAAGAA5830.10107717406945718No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5830.10107717406945718No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5790.10038367716332025No Hit
GTACATCAGGCGGAGAAGCGGGCGGTAGAGCCCTAAGCGGCCCCGGGAGG5770.10003692871025179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)