FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539554
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA35360.6553560904005902No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC32110.5951211556211241No Hit
CGGTGGTCTCCATGTAACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG16810.3115536165054842No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGATGAAAGTCCAA15400.2854209217242389No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14350.2659604043339499No Hit
ACTCCTGCTCAAGGAACCTCTACGTTTCCCTCATGTTGCTGTACAAAACC14240.263921683464491No Hit
GTTTTAGGAAACTCCCTGTAAACCGGCCTATTGATTGGAAAGTGTGTCAA13550.25113334346515825No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATACCCACATTGTGTGAAA13510.25039199042171867No Hit
CCTTAGGATAGAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCA12820.2376036504223859No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGCTATTCCCTTAACTTCAT12810.237418312161526No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCATCCACCAATCGGCAGG11070.2051694547719042No Hit
TCCTAAAACAGTGTTTGATTTTCAGCACAATATGTTCCTGGGGTAAGGTG10830.2007213365112667No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG10650.1973852478157886No Hit
ATATGTTCCTGGGGTAAGGTGCCCCAACTTCCAATTACATATCCCATGAA10230.189601040859673No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTATAAGGCC10050.18626495216419486No Hit
GCCTAGCAGCCATGGAAAAGATGTATATTTGCGGGAAAGGACGACAGAAT10010.18552359912075528No Hit
GAGCAGTAGTCGTGCAGGTTCTGCATGGTCCGGTGCTGGTGGTTGATGAT9840.18237284868613707No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9780.1812608191209777No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9610.17811006868635948No Hit
CTTATAAGGCCTTTCTACACAAACAATATCTGAACCTTTACCCCGTTGCT9120.1690284939042246No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGATGAAAGTCCAA9030.16736044955648555No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA9010.16698977303476575No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8940.16569240520874648No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG8880.1645803756435871No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8760.16235631651326837No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8730.16180030173068868No Hit
CCTCTACGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8710.1614296252089689No Hit
ACATCGATGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTG8670.1606882721655293No Hit
ATCCTAAGGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT8530.15809353651349076No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8440.15642549216575172No Hit
GTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCGTTGACACACT8230.15253338868769392No Hit
ATATAAAAGAGAGACAACACATAGCGCCTCATTTTGCGGGTCACCATATT8120.15049466781823506No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8050.1491972999922158No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCATTCAC7990.1480852704270564No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7930.14697324086189706No Hit
GGATAATAAGGTTTAATTCCTTTATCTAAGGGCAAATATTTAGTAACCTT7840.145305196514158No Hit
GGGTAAAGGTTCAGATATTGTTTGTGTAGAAAGGCCTTATAAGTTGGCGA7760.14382249042727882No Hit
TTATTATCCAGAGCATGTGGTTAATCATTACTTCCAGACAAGACATTATT7580.14048640173180071No Hit
GCCTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTCACTTT7410.13733565129718248No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7330.13585294521030333No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7330.13585294521030333No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG7330.13585294521030333No Hit
GTTAATAATGTCTTCCTGCAAATGAATGTCCGGAAAAGAAGGAGTTTGCC7300.13529693042772364No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTACCCCAG7290.13511159216686375No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7180.1330728712974049No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7130.13214617999310543No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6990.12955144434106688No Hit
ATGTGGGTATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCTAAAC6860.12714204694988823No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6830.12658603216730854No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6810.12621535564558878No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6780.1256593408630091No Hit
GCTCTGTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6760.12528866434128927No Hit
AACTTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCA6740.1249179878195695No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCAGGTCTGGAGC6720.1245473112978497No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT6660.12343528173269033No Hit
TACCCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCG6650.12324994347183042No Hit
AACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGC6640.12306460521097054No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATACCTATGGGAG6610.12250859042839085No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6530.1210258843415117No Hit
GTTCAGACTACTGCCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6470.11991385477635232No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6450.11954317825463254No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTG6440.11935783999377264No Hit
CTGCACGTTACATGGAGACCACCGTGAACGCCCACCGCAACCTGCCCAAG6420.11898716347205285No Hit
GATGTATATTTGCGGGAAAGGACGACAGAATTGTCGGTCCCAATGAGTTT6370.11806047216775337No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6330.1173191191243138No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCC6120.11342701564625599No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6090.1128710008636763No Hit
ATATACATCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6070.11250032434195649No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG6060.11231498608109661No Hit
GTTTACACCTGTTAATAATGTCTTCCTGCAAATGAATGTCCGGAAAAGAA6020.11157363303765702No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTG6020.11157363303765702No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5900.1093495739073383No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACCACA5870.1087935591247586No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC5840.10823754434217892No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5810.10768152955959923No Hit
CATATAAAGGCATTAAGGCAGGATACCCACATTGTGTGAAAGGAGCAGCA5790.10731085303787943No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGA5730.10619882347272006No Hit
GTACAGTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5700.10564280869014037No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5700.10564280869014037No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT5660.1049014556467008No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5610.10397476434240133No Hit
GTGTTGGGGGGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5580.10341874955982164No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC5540.10267739651638205No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGACTGAGATCT5500.10193604347294247No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5480.10156536695122267No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5450.10100935216864299No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5400.10008266086434352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)