FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465593
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14090.30262482468593815No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC13770.29575186912174367No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG12510.2686896065877279No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12070.2592392926869605No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11880.25515847532072No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11650.25021853850895526No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCA11220.24098300446956888No Hit
GTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG9560.20532954748031007No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCCAGG9190.1973826926092102No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG9060.19459055441125617No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8980.19287231552020756No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA8610.1849254606491077No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA8560.1838515613422023No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCAC8450.18148898286701046No Hit
GGTTAGGATAGAACCTGGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8220.17654904605524568No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8150.17504558702557815No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8020.1722534488276241No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7570.16258835506547564No Hit
ATTTACAGGAAGTTTCCGAAAACATTGCTTCAATTTTTGTACAATATGTT7370.15829275783785407No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7350.15786319811509195No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7270.1561449592240433No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7260.15593017936266224No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA7070.15184936199642177No Hit
CCAATCGGCAGTCAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGA6960.14948678352122993No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6940.14905722379846775No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC6910.14841288421432453No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6770.14540596615498944No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6680.14347294740255975No Hit
GTCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTGTGG6680.14347294740255975No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC6500.13960690989770036No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6360.13659999183836527No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA6310.1355260925314599No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6250.1342374133631734No Hit
GGCCTATACTTTCCTGCTGGTGGCTCAAGTTCCGGAACAGTAAGCCCTGT6170.13251917447212477No Hit
CTCCAAAAGACACCAAATACTCTAAAACAGTTTCTCTTCCAAAAGTAAGA6000.12886791682864648No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5920.12714967793759788No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5860.1258609987693114No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCACGCAGCGCC5800.12457231960102493No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG5800.12457231960102493No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5680.12199496126445201No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCACGCAGCGCCTCAT5640.12113584181892768No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5630.12092106195754661No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5580.11984716265064123No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5550.11920282306649799No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5530.11877326334373582No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT5510.11834370362097368No Hit
CGGTACAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGAGAGACAG5500.11812892375959261No Hit
ATATAAGAGAGAAACTGCACGCAGCGCCTCATTTTGTGGGTCACCATATT5390.11576634528440075No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5380.11555156542301967No Hit
TGGTAATAGAGGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCC5330.11447766611611429No Hit
CTCTAAAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5280.11340376680920891No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGCTTACTGTTCCGGAACTTGAG5200.11168552791816029No Hit
GGATAGAACCTGGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC5170.11104118833401705No Hit
TGGTTATAGAGGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCC5160.11082640847263597No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5110.1097525091657306No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG5060.10867860985882519No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5060.10867860985882519No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5030.10803427027468197No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT5030.10803427027468197No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5010.10760471055191981No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCACGC5010.10760471055191981No Hit
TCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTAGTTG4960.10653081124501443No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4950.10631603138363335No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4940.10610125152225228No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4910.10545691193810904No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4910.10545691193810904No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4860.10438301263120366No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4830.10373867304706041No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGGGCTTT4810.10330911332429828No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4790.10287955360153611No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4720.10137609457186857No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4710.10116131471048749No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4700.10094653484910641No Hit
CTCCATATTCGGTACAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4690.10073175498772532No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC4690.10073175498772532No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4680.10051697512634425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)