FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465593
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13550.29102671217135995No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG13220.2839389767457844No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11880.25515847532072No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11790.2532254565682903No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11590.2489298593406688No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11140.2392647655785203No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCA10540.22637797389565567No Hit
GTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG9630.2068330065099776No Hit
GGTTAGGATAGAACCTGGCAGGCATAATCAATTTTAATCTCCTTTTTTCG9450.2029669690051182No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9300.19974527108440207No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA9150.19652357316368588No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCCAGG8970.19265753565882648No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG8720.18728803912429953No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA8430.1810594231442483No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7740.16623961270895396No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7340.15764841825371087No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA7260.15593017936266224No Hit
ATTTACAGGAAGTTTCCGAAAACATTGCTTCAATTTTTGTACAATATGTT7260.15593017936266224No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA6780.14562074601637054No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCAC6750.14497640643222728No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC6750.14497640643222728No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6730.14454684670946513No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6640.14261382795703545No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6590.14153992865013004No Hit
GTCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTGTGG6560.14089558906598681No Hit
CCAATCGGCAGTCAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGA6540.14046602934322466No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6420.13788867100665173No Hit
CTCCAAAAGACACCAAATACTCTAAAACAGTTTCTCTTCCAAAAGTAAGA6390.1372443314225085No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6370.13681477169974635No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6320.13574087239284097No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA6110.13123049530383832No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6000.12886791682864648No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5940.12757923766036No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCACGCAGCGCC5880.12629055849207355No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5870.12607577863069247No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5840.12543143904654924No Hit
GGATAGAACCTGGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC5810.12478709946240601No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5720.1228540807099763No Hit
GGCCTATACTTTCCTGCTGGTGGCTCAAGTTCCGGAACAGTAAGCCCTGT5670.12178018140307093No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5640.12113584181892768No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG5570.11963238278926015No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5480.11769936403683044No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5470.11748458417544937No Hit
CGGTACAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGAGAGACAG5390.11576634528440075No Hit
CTCTAAAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5340.11469244597749537No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5330.11447766611611429No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5310.11404810639335212No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5270.11318898694782784No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5270.11318898694782784No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGGGCTTT5270.11318898694782784No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGCTTACTGTTCCGGAACTTGAG5260.11297420708644675No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5250.11275942722506567No Hit
GTTTTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC5210.11190030777954137No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5140.11039684874987381No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5100.1095377293043495No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTGCACGCAGCGCCTCAT5070.10889338972020626No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5070.10889338972020626No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT5060.10867860985882519No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5060.10867860985882519No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5050.10846382999744413No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5000.10738993069053873No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4960.10653081124501443No Hit
TGGTAATAGAGGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCC4950.10631603138363335No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4910.10545691193810904No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4900.10524213207672795No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4890.1050273522153469No Hit
TGGTTATAGAGGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCC4890.1050273522153469No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT4880.10481257235396581No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4780.10266477374015504No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4730.10159087443324966No Hit
ATATGTTCTTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4720.10137609457186857No Hit
CTCCATATTCGGTACAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4700.10094653484910641No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC4690.10073175498772532No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4680.10051697512634425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)