FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516181
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG18100.3506521937072461No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGGTG16930.3279857259372197No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA16100.3119060949550642No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15350.297376307922996No Hit
CTATCGATAATATCCTCTTTCAAGTGAATGTGAGGAAAAGAGGGAGTTTT15170.29388915903529966No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC14620.2832339818784496No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14330.2776157975593832No Hit
GTATGTAAATAATGCCTCGTTTGGAAGTAATGATTAACTATATGTTCAGG13560.2626985495397932No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13260.25688663472696593No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12940.2506872589266168No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT11650.2256960252314595No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC10710.207485358817934No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG10500.2034170184489549No Hit
CCCTTTACCCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9640.1867561959855167No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9550.18501262154166853No Hit
CCCTATTCTTGGGAACAAGAGCTACAGCAAACCTCGACAAGGCATGGGGA9430.1826878556165376No Hit
GTGCTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGG9390.18191293364149397No Hit
ATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATTAAACCTTAT9260.17939443722260215No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8800.17048283450960033No Hit
GGGCACATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATC8710.16873926006575213No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT8670.1679643380907085No Hit
GAATATGGTGACCCACAAAATGAGGCGCTGCGAGTAGTTTCTCTCTTATA8440.16350853673420757No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8270.1602151183402721No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG8130.1575028914276194No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG7950.154015742539923No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGGGTAAAG7910.15324082056487937No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7660.14839755822085662No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7620.147622636245813No Hit
GGGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACC7610.1474289057520521No Hit
CTCCACCACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTAT7500.1452978703206821No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7370.14277937390179024No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG7330.14200445192674663No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCGTTAAC7290.141229529951703No Hit
ACCCTATTCTTGGGAACAAGAGCTACAGCAAACCTCGACAAGGCATGGGG7180.139098494520333No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7130.13812984205152845No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7040.13638626760768024No Hit
GATCACTGGCCAGCGGCAAACCAGGTAGGAGTGGGATCATTCGGGCCAGG7030.13619253711391932No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGAAAACTCCCTCTTTTCCTCAC6920.13406150168254932No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6910.13386777118878843No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCAAATTTTGGCCAGGACACG6900.13367404069502753No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6870.13309284921374478No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG6860.1328991187199839No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6810.13193046625117932No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA6780.1313492747698966No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6770.13115554427613568No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6730.13038062230109204No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6600.12786212588220025No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT6600.12786212588220025No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATATTTGGTAAGGTT6560.1270872039071566No Hit
CCATACTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA6460.1251498989695475No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6420.12437497699450387No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6400.12398751600698206No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6350.12301886353817751No Hit
CTATTGATAATATCCTCTTTCAAGTGAATGTGAGGAAAAGAGGGAGTTTT6350.12301886353817751No Hit
GAATAGGGTGACCCACAAAATGAGGCGCTGCGAGTAGTTTCTCTCTTATA6340.12282513304441658No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6330.12263140255065567No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6320.12243767205689478No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT6200.12011290613176387No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTA6150.11914425366295932No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6150.11914425366295932No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6080.11778814020663295No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA6000.11623829625654566No Hit
CCTATAAATAGACCTATTGATTGGAAGGTATGTCAAAGAATTGTGGGTCT5980.11585083526902384No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5980.11585083526902384No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5940.1150759132939802No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATATTTGGTA5900.11430099131893656No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5870.11371979983765385No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCTTTAGATAAAGGCATTAAAC5860.11352606934389295No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5830.1129448778626102No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA5780.11197622539380567No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5780.11197622539380567No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTATTTGCCAAGTGTTTGCTG5770.11178249490004476No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5750.11139503391252292No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5730.11100757292500112No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5720.1108138424312402No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG5680.11003892045619657No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG5670.10984518996243567No Hit
GACTTCTTTCCTTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTA5620.10887653749363112No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT5530.10713296304978293No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5500.1065517715685002No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5480.10616431058097839No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACGAGGCATTATT5470.10597058008721748No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5460.10577684959345655No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5460.10577684959345655No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5430.10519565811217384No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5340.10345208366832565No Hit
TCCACAGAGTATGTAAATAATGCCTCGTTTGGAAGTAATGATTAACTATA5330.10325835317456473No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5320.10306462268080384No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5260.10190223971823836No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5230.10132104823695565No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5220.10112731774319474No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5190.100546126261912No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5180.1003523957681511No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5170.1001586652743902No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5170.1001586652743902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)