FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241212
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCAC16560.6865330083080444No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11210.4647364144404093No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10000.41457307264978527No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA8290.34368107722667196No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC8230.3411936387907733No Hit
CCTTTATGGGGTTGAAGTCCCAGTCTGGCTTGTTTGAATTGGCTCCGAAT7980.33082931197452864No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6940.28771371241895094No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG6800.281909689401854No Hit
CCATCAAGGACCACTGGCCACAGGCCAACCAGGTAGGAGTGGGAGCATTC6780.2810805432565544No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6260.2595227434787656No Hit
GGAATTCCACAGCTTTCCACCAAGCTCTGCAAGATCCCAGAGTCAGGGGC6250.2591081704061158No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT6100.252889574316369No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA5990.24832927051722137No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5550.23008805532063079No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGGTAACCACATTGTGTAAAT5420.2246986053761836No Hit
GTACCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG5380.22304031308558445No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTCT5270.21848000928643682No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5270.21848000928643682No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA4800.1989950748718969No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4690.19443477107274929No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC4660.19319105185479993No Hit
GCATAGTATGGCTTGCCTGAGTGCTGTATGGTGAGGTGAGCAATGCTCAG4600.1907036134189012No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA4590.19028904034625144No Hit
ATTAATAGGTGTCAGCAATTTGTGGGCCCTCTCACTGTAAATGAAAAAAG4510.18697245576505317No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4300.17826642123940764No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG4240.17577898280350895No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4220.17494983665820937No Hit
CCTATTAATAATGTCCTCATGTAAATGGATTTTAGGAAAGGAAGGAGTTT4220.17494983665820937No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCTCACCCGTG4190.17370611744026002No Hit
CCATTTACACAATGTGGTTACCCTGCCTTAATGCCTTTGTATGCATGTAT4020.16665837520521365No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG3890.16126892526076647No Hit
GGGGCCTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGA3860.16002520604281711No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTCCG3830.15878148682486776No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT3780.15670862146161882No Hit
CCATAAAGGACCACTGGCCACAGGCCAACCAGGTAGGAGTGGGAGCATTC3730.15463575609836988No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTCGAAAACTTCCTGTCA3700.15339203688042055No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA3560.14758801386332354No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGCTATTCCCTAAACTTCAT3550.14717344079067377No Hit
GTGGCCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAACACCTCCGTG3530.14634429464537418No Hit
GTGCTGGTGGGAGTGTCTTTTAGCATGCTGATGCATTGTAATTAGCATAC3520.1459297215727244No Hit
ATTGACCACAGTGCCAACAGTTCCTCCTCCTGCCTCCACCAATCGGCAGT3500.14510057542742483No Hit
CATGTATACAGGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3480.14427142928212525No Hit
CCTTGATGGGGTTGAAGTCCCAGTCTGGCTTGTTTGAATTGGCTCCGAAT3340.13846740626502826No Hit
TTATTATCCTGATCAAGTAGTTAATCATTACTTCCAGACCAGACATTATT3270.13556539475647977No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC3260.13515082168383No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3190.13224881017528148No Hit
GATGTCGAGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGC3170.13141966402998193No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3170.13141966402998193No Hit
GTGCTGGTGGACTATTAGAGGAGGAAGCCAAGCTACAGCAAAGCTGTTGA3140.13017594481203257No Hit
ATTTATGCCTACAGCCTCCTAATACATAAATCTTTAACCTAATCTCCTCC3050.1264447871581845No Hit
GGAAGATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC3050.1264447871581845No Hit
CCCTAGCAGCCATGGAAATGATGTATATTTCCGAGAAAGGACGACAGAAT3030.12561564101288494No Hit
TCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAG3020.12520106794023514No Hit
GTACGGGACCCTGCAGGACCTGCACGACTCCTGCTCAAGGCAACTCTATG2950.12229905643168665No Hit
ACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA2930.12146991028638707No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG2930.12146991028638707No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG2920.12105533721373729No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG2900.12022619106843771No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA2900.12022619106843771No Hit
GTCCAATAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC2870.11898247185048838No Hit
TGATGATGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAA2870.11898247185048838No Hit
GGCTGGAGGAGTGCGGATCCACACTCCGAAGGAGACCAAATACTCAAGGA2860.11856789877783859No Hit
TCCCGATACAGAGCTGAGGCTGTATCAAGTAGATCCCGGACTGAAGGAAA2830.11732417955988923No Hit
ATGATGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC2820.11690960648723944No Hit
GTGCTGGTGTGTACTGCCAACTGGATTCTTCGCGGGACGTCCTTTGTTTA2780.11525131419664031No Hit
GGACCTGCACGACTCCTGCTCAAGGCAACTCTATGTTTCCCTCATGTTGC2770.11483674112399052No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA2770.11483674112399052No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA2750.11400759497869094No Hit
ATACTATGCTGGGGGGAATTAATGACTCTAGCTACCTGGGTGGGTAATAA2710.1123493026880918No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG2710.1123493026880918No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG2660.11027643732484287No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2650.10986186425219309No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC2650.10986186425219309No Hit
GGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGCTATTCCCTAA2640.1094472911795433No Hit
CCTAATACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC2600.10778899888894418No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTACT2550.10571613352569524No Hit
GGCCTAAAGATCAGACAACTATTGTGGTTTCATATTTCTTGCCTTACTTT2540.10530156045304546No Hit
CTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCA2540.10530156045304546No Hit
ATGTTAGTGTTAACATAATTGACTACTAGATCCCTGGATGCAGGATCTTG2530.10488698738039567No Hit
CCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCC2520.10447241430774588No Hit
GCCTTAATGCCTTTGTATGCATGTATACAGGCGAAACAGGCTTTTACTTT2510.1040578412350961No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA2500.10364326816244632No Hit
GTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATAGGC2440.1011558297265476No Hit
GACTCTTGGACTCCCAGCAATGTCAACGACCGACCTTGAGGCCTACTTCA2430.10074125665389781No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC2430.10074125665389781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)