FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241212
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13710.5683796826028555No Hit
CCAATAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCAC11410.47302787589340495No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC10000.41457307264978527No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC8370.34699766180787023No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA7980.33082931197452864No Hit
CCTTTATGGGGTTGAAGTCCCAGTCTGGCTTGTTTGAATTGGCTCCGAAT7770.32212327744888314No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG7730.320464985158284No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7630.31631925443178616No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7010.29061572392749946No Hit
CCATCAAGGACCACTGGCCACAGGCCAACCAGGTAGGAGTGGGAGCATTC6180.2562061588975673No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5920.24542725900867285No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT5890.24418353979072352No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA5220.2164071439231879No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGGTAACCACATTGTGTAAAT5160.2139197054872892No Hit
GTACCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG5060.20977397476079135No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA5040.20894482861549177No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTCT4780.19816592872659733No Hit
CCATTTACACAATGTGGTTACCCTGCCTTAATGCCTTTGTATGCATGTAT4660.19319105185479993No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4660.19319105185479993No Hit
GTGCTGGTGTGTACTGCCAACTGGATTCTTCGCGGGACGTCCTTTGTTTA4650.19277647878215015No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA4610.191118186491551No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTCCG4560.18904532112830205No Hit
GGAATTCCACAGCTTTCCACCAAGCTCTGCAAGATCCCAGAGTCAGGGGC4490.1861433096197536No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG4470.185314163474454No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCTCACCCGTG4440.18407044425650465No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4390.1819975788932557No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4370.18116843274795616No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC4220.17494983665820937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4210.1745352635855596No Hit
ATTAATAGGTGTCAGCAATTTGTGGGCCCTCTCACTGTAAATGAAAAAAG4210.1745352635855596No Hit
CCTATTAATAATGTCCTCATGTAAATGGATTTTAGGAAAGGAAGGAGTTT4070.1687312405684626No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTCGAAAACTTCCTGTCA3960.16417093676931496No Hit
CCTTGATGGGGTTGAAGTCCCAGTCTGGCTTGTTTGAATTGGCTCCGAAT3830.15878148682486776No Hit
CCATAAAGGACCACTGGCCACAGGCCAACCAGGTAGGAGTGGGAGCATTC3690.15297746380777077No Hit
GCATAGTATGGCTTGCCTGAGTGCTGTATGGTGAGGTGAGCAATGCTCAG3480.14427142928212525No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3460.1434422831368257No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT3380.14012569855562743No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA3320.1376382601197287No Hit
GTGCTGGTGGGAGTGTCTTTTAGCATGCTGATGCATTGTAATTAGCATAC3300.13680911397442913No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGCTATTCCCTAAACTTCAT3280.13597996782912955No Hit
GGGGCCTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGA3280.13597996782912955No Hit
GTACGGGACCCTGCAGGACCTGCACGACTCCTGCTCAAGGCAACTCTATG3260.13515082168383No Hit
GGCTGGAGGAGTGCGGATCCACACTCCGAAGGAGACCAAATACTCAAGGA3200.13266338324793128No Hit
ATTGACCACAGTGCCAACAGTTCCTCCTCCTGCCTCCACCAATCGGCAGT3180.1318342371026317No Hit
CCCTAGCAGCCATGGAAATGATGTATATTTCCGAGAAAGGACGACAGAAT3160.13100509095733215No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG3100.12851765252143343No Hit
TTATTATCCTGATCAAGTAGTTAATCATTACTTCCAGACCAGACATTATT3070.12727393330348408No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3040.12603021408553472No Hit
CATGTATACAGGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC2980.123542775649636No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC2960.12271362950433642No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2960.12271362950433642No Hit
GTGCTGGTGGACTATTAGAGGAGGAAGCCAAGCTACAGCAAAGCTGTTGA2950.12229905643168665No Hit
GTGGCCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAACACCTCCGTG2940.12188448335903686No Hit
CCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCC2890.11981161799578795No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG2880.11939704492313816No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC2830.11732417955988923No Hit
GGAAGATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC2810.11649503341458967No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG2800.11608046034193988No Hit
GCCTTAATGCCTTTGTATGCATGTATACAGGCGAAACAGGCTTTTACTTT2780.11525131419664031No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG2710.1123493026880918No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA2700.11193472961544201No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT2630.10903271810689351No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA2630.10903271810689351No Hit
ATAGAGACCCAAGCGACCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG2620.10861814503424372No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA2620.10861814503424372No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG2620.10861814503424372No Hit
ATTTATGCCTACAGCCTCCTAATACATAAATCTTTAACCTAATCTCCTCC2600.10778899888894418No Hit
GATGTCGAGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGC2600.10778899888894418No Hit
GACTCTTGGACTCCCAGCAATGTCAACGACCGACCTTGAGGCCTACTTCA2550.10571613352569524No Hit
ACATAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA2550.10571613352569524No Hit
GTCCAATAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC2520.10447241430774588No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2510.1040578412350961No Hit
GTATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG2510.1040578412350961No Hit
GTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTACCCCAACTTC2500.10364326816244632No Hit
TCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAG2490.10322869508979653No Hit
ATGATGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC2490.10322869508979653No Hit
ATACTATGCTGGGGGGAATTAATGACTCTAGCTACCTGGGTGGGTAATAA2480.10281412201714674No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG2470.10239954894449696No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG2460.10198497587184717No Hit
GGACCTGCACGACTCCTGCTCAAGGCAACTCTATGTTTCCCTCATGTTGC2450.10157040279919738No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA2450.10157040279919738No Hit
TGATGATGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAA2420.10032668358124802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)