FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238956
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19300.8076800749928857No Hit
CCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA19220.8043321783089774No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA18120.7582985989052378No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC12160.5088802959540668No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC11950.5000920671588075No Hit
CTGTTGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAGA11410.47749376454242626No Hit
GTACAGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA11180.4678685615761897No Hit
CTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCA9080.37998627362359594No Hit
CCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGAGC8070.33771907798925327No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8060.3373005909037647No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7760.32474597833910845No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA6400.26783173471266675No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6240.2611359413448501No Hit
CCTCCTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT6130.25653258340447616No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6030.2523477125495907No Hit
ATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAA5810.24314099666884278No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTGCGTCTGCGAGGCGA5780.24188553541237717No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5680.23770066455749173No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5640.2360267162155376No Hit
GGTTTAAGTCCCAATCTGGATTGTTTGAGTTGGCTCCGAATGCAGGGTCC5610.23477125495907197No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA5580.23351579370260636No Hit
CCTCCACACGGCGGTATTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATT5480.2293309228477209No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5350.22389059073636988No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5290.22137966822343863No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5200.21761328445404177No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA5160.21593933611208757No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG5160.21593933611208757No Hit
GGAAGATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC5050.21133597817171362No Hit
GTGTAGAGGGGTGAACCCTGGCCCGAATGCTCCCACTCCTACCTGGTTGG4960.20756959440231676No Hit
CGGGCAACATACCTTGATAATCCAGAAGAACCAATAAGAAGATGAGGCAT4840.20254774937645426No Hit
CTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCTTG4720.19752590435059172No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT4700.19668893017961464No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG4660.1950149818376605No Hit
TATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATG4620.1933410334957063No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT4600.19250405932472925No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTTGAGGACAAACGGGTAAC4530.18957464972630944No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4470.1870637272133782No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4310.18036793384556152No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT4160.1740906275632334No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG4130.17283516630676776No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA4080.17074273087932507No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4050.16948726962285943No Hit
GTACAATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCC4000.16739483419541673No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT3960.16572088585346256No Hit
ACTTCAACCCCATCAAGGACCACTGGCCAGCAGCCAACCAGGTAGGAGTG3940.16488391168248548No Hit
GTAGATGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3860.16153601499857714No Hit
CCTATTCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA3830.1602805537421115No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3780.1581881183146688No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT3720.15567719580173756No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT3700.15484022163076047No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3680.1540032474597834No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3590.15023686369038652No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT3550.14856291534843233No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA3550.14856291534843233No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3530.14772594117745527No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT3510.1468889670064782No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTTACTTT3500.14647047992098963No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAGGAGGTTGGTGAGTGAT3470.14521501866452402No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3440.1439595574080584No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT3410.14270409615159277No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG3380.14144863489512713No Hit
CCTAGTCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA3370.1410301478096386No Hit
GTATGTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCTCCATTTACACA3270.13684527695475318No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGTAATGTTCCCCAACTTC3250.1360083027837761No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA3240.13558981569828757No Hit
AGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGG3190.13349738027084485No Hit
GGCTCCGAGGGGCGTTCACGGTGGTCTCCATGCAACGTGCAGAGGTGAAG3170.13266040609986776No Hit
CCTCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGAT3170.13266040609986776No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC3170.13266040609986776No Hit
GTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC3160.13224191901437923No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT3080.1288940223304709No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGT3070.12847553524498234No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT3060.12805704815949379No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3050.12763856107400526No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3010.1259646127320511No Hit
ATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCTCGATACAGAG3010.1259646127320511No Hit
GCTCATCGGCACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT2980.12470915147558545No Hit
GTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAAGTTTAGGGAA2950.12345369021911984No Hit
CTAAATAAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG2910.12177974187716567No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG2900.12136125479167714No Hit
GACTTCAACCCCATCAAGGACCACTGGCCAGCAGCCAACCAGGTAGGAGT2880.12052428062070004No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT2880.12052428062070004No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2880.12052428062070004No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG2830.11843184519325733No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA2820.1180133581077688No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG2790.11675789685130318No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA2760.11550243559483755No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC2740.11466546142386046No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2730.1142469743383719No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT2710.11341000016739484No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA2710.11341000016739484No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA2710.11341000016739484No Hit
GCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCT2690.11257302599641775No Hit
GTTTAATCTTTTGTACAATATGATCCTGTGGTAATGTTCCCCAACTTCCA2660.11131756473995212No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG2650.1108990776544636No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG2610.10922512931250941No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT2590.10838815514153234No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT2520.10545874554311253No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC2510.105040258457624No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG2490.10420328428664691No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT2480.10378479720115838No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC2460.1029478230301813No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG2460.1029478230301813No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGCCTC2430.10169236177371567No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC2410.10085538760273857No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT2390.10001841343176149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)