FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238956
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22040.9223455364167462No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA18820.7875926948894357No Hit
CTGTTGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAGA12710.5318970856559367No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC11090.4641021778067929No Hit
GTACAGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA10070.4214164950869616No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC9730.4071879341803512No Hit
CCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA9680.4050954987529085No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7620.3188871591422689No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7470.31260985285994075No Hit
CCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGAGC7430.3109359045179866No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGTAATGTTCCCCAACTTC6800.2845712181322084No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6780.28373424396123137No Hit
CTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCA6660.27871239893536887No Hit
ATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAA6290.2632283767722928No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6080.2544401479770334No Hit
CTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCTTG5890.24648889335275115No Hit
CCTCCTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT5870.24565191918177404No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTGCGTCTGCGAGGCGA5840.24439645792530842No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5730.23979309998493448No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG5720.23937461289944592No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA5650.2364452033010261No Hit
GGTTTAAGTCCCAATCTGGATTGTTTGAGTTGGCTCCGAATGCAGGGTCC5450.2280754615912553No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5290.22137966822343863No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5200.21761328445404177No Hit
CCTCCACACGGCGGTATTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATT5200.21761328445404177No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5130.21468387485562196No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG5090.21300992651366776No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5070.2121729523426907No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT5000.2092435427442709No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA4880.2042216977184084No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT4710.19710741726510322No Hit
GGAAGATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC4710.19710741726510322No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4700.19668893017961464No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG4700.19668893017961464No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4700.19668893017961464No Hit
GGTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGT4650.19459649475217194No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4480.18748221429886672No Hit
GTGTAGAGGGGTGAACCCTGGCCCGAATGCTCCCACTCCTACCTGGTTGG4390.18371583052946985No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4340.18162339510202713No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT4280.1791124725890959No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4230.1770200371616532No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT4160.1740906275632334No Hit
GTACAATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCC4150.17367214047774487No Hit
TATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATG4070.17032424379383654No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG3950.16530239876797403No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3940.16488391168248548No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTTGAGGACAAACGGGTAAC3890.16279147625504276No Hit
GTAGATGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3840.16069904082760006No Hit
ACTTCAACCCCATCAAGGACCACTGGCCAGCAGCCAACCAGGTAGGAGTG3840.16069904082760006No Hit
AGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGG3830.1602805537421115No Hit
GGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACGGGGCG3820.15986206665662298No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT3800.1590250924856459No Hit
CGGGCAACATACCTTGATAATCCAGAAGAACCAATAAGAAGATGAGGCAT3690.15442173454527192No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT3660.1531662732888063No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3660.1531662732888063No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA3560.14898140243392088No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3550.14856291534843233No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA3510.1468889670064782No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG3480.14563350575001255No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3460.14479653157903546No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3450.14437804449354694No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC3350.1401931736386615No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3310.13851922529670735No Hit
CCTCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGAT3300.13810073821121882No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3300.13810073821121882No Hit
CTAAATAAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG3250.1360083027837761No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT3240.13558981569828757No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAGGAGGTTGGTGAGTGAT3130.1309864577579136No Hit
GTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC3120.13056797067242504No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA3110.1301494835869365No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTTACTTT3100.12973099650144798No Hit
GTATGTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCTCCATTTACACA3060.12805704815949379No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG3020.12638309981753965No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC3010.1259646127320511No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT2990.125127638561074No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT2960.1238721773046084No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG2910.12177974187716567No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG2860.11968730644972296No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT2850.11926881936423442No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGT2840.11885033227874586No Hit
GCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCT2740.11466546142386046No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA2730.1142469743383719No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA2690.11257302599641775No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT2680.11215453891092922No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT2660.11131756473995212No Hit
GTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAAGTTTAGGGAA2600.10880664222702088No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT2600.10880664222702088No Hit
AGAAAGTCCCAAGCGGCCCCGAGAGGGGTCGTCCGCGGGATTCAGCGCCG2590.10838815514153234No Hit
CCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCTTCTGGATT2580.10796966805604379No Hit
GCTCATCGGCACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT2560.1071326938850667No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC2560.1071326938850667No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT2540.10629571971408963No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC2520.10545874554311253No Hit
CCTATTCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA2490.10420328428664691No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA2490.10420328428664691No Hit
GCATGACCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC2480.10378479720115838No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA2480.10378479720115838No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGGCCCTTTATACC2470.10336631011566982No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT2460.1029478230301813No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC2450.10252933594469275No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT2430.10169236177371567No Hit
CCTAGTCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA2420.10127387468822713No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG2410.10085538760273857No Hit
ACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAAC2400.10043690051725004No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT2390.10001841343176149No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC2390.10001841343176149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)