FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421946
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA26850.6363373512250383No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG16810.39839221132561986No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14820.3512297782180658No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC14400.3412758978637077No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG14020.3322700061145265No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT13600.3223161257601683No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT12730.3016973735975694No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA12640.2995643992359212No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12030.2851075730069724No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG11100.263066837936608No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAGCCCATGAA10970.25998587496978287No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT10630.2519279718257787No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10380.24600304304342263No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAA10180.2412631000175378No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC9590.22728026809117752No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAAGTAGAGGACAAACGGGCAAC9260.2194593620984676No Hit
GGCAACTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACT9060.21471941907258274No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8930.2116384561057576No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8770.20784650168504973No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA8430.19978859854104555No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC8150.19315267830480679No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7790.18462078085821407No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7780.18438378370691982No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC7690.18225080934527169No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG7490.17751086631938684No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7350.17419290620126746No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7340.1739559090499732No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT7310.1732449175960905No Hit
TCTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGAT7240.1715859375370308No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA7240.1715859375370308No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7160.16968996032667688No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7150.16945296317538264No Hit
GGATCATATTGTACAAAAGATCAGACACTGTTTTAGAAAACTTCCTGTTA7130.16897896887279415No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7120.1687419717214999No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6980.16542401160338055No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG6910.16376503154432082No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATCAAACCTTATTATCCAGATC6890.16329103724173236No Hit
GTACTGTAGTGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6770.16044707142620146No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6760.16021007427490722No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6750.15997307712361297No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6730.15949908282102448No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6730.15949908282102448No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6580.15594412555161086No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6470.1533371568873742No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6310.14954520246666633No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6220.1474122281050182No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6080.14409426798689878No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT5930.14053931071748516No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5910.1400653164148967No Hit
CTCTACTTCCAGGATCAACAACAACCAGTACGGGACCATGCAAAACCTGC5830.13816933920454277No Hit
GGATTAAAGATAGGTACTGTAGTGGAATAAAGCCCAGTAAAGTTTCCCAC5830.13816933920454277No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5820.1379323420532485No Hit
GGATAATAAGGTTTGATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5790.1372213505993658No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5690.1348513790864234No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5570.1320074132708925No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT5570.1320074132708925No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA5550.131533418968304No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5530.13105942466571552No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAAT5510.13058543036312703No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5410.12821545885018462No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5390.12774146454759613No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5370.12726747024500765No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTCTGATCTTTTGT5340.12655647879112494No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5340.12655647879112494No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC5300.12560849018594797No Hit
GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCC5290.12537149303465372No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5260.124660501580771No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5190.12300152152171132No Hit
GTAGTGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5150.12205353291653434No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5120.12134254146265162No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5050.11968356140359193No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5020.1189725699497092No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT5010.11873557279841497No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5010.11873557279841497No Hit
GTTATGGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGG4990.11826157849582647No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4950.11731358989064951No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4950.11731358989064951No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAA4940.11707659273935528No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC4890.11589160698288407No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4870.11541761268029557No Hit
GCACTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACCCTCTGGGA4820.11423262692382438No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT4800.1137586326212359No Hit
AACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCATGGGCTA4780.11328463831864742No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC4770.11304764116735316No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4770.11304764116735316No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4760.11281064401605892No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4760.11281064401605892No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG4750.11257364686476469No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4740.11233664971347045No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC4730.1120996525621762No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA4710.11162565825958773No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4700.11138866110829349No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4690.11115166395699924No Hit
TTCTAAAACAGTGTCTGATCTTTTGTACAATATGATCCTGTGGCAATGTT4670.11067766965441077No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATCAAAC4640.10996667820052802No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG4600.10901868959535105No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4580.10854469529276257No Hit
ACATAATTGGAAGTTGGGGAACATTGCCACAGGATCATATTGTACAAAAG4570.10830769814146833No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4560.1080707009901741No Hit
ATAGTCGCCTGATCGTTAAACCCATGTTAGTATTAACATAATTGACTACT4560.1080707009901741No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4560.1080707009901741No Hit
ATCTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGA4550.10783370383887986No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT4530.10735970953629137No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC4450.10546373232593743No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAGCCC4440.1052267351746432No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA4390.10404174941817199No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT4360.10333075796428927No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC4310.10214577220781806No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4300.1019087750565238No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA4280.10143478075393533No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC4270.10119778360264109No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG4240.10048679214875837No Hit
TCTACTTCCAGGATCAACAACAACCAGTACGGGACCATGCAAAACCTGCA4220.1000127978461699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)