FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421946
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA29740.704829527949074No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG17320.4104790660416262No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG13910.32966303745028985No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT13140.3114142568006332No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13040.30904428528769085No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12890.3054893280182772No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA12750.30217136790015786No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC12660.3000383935385097No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA12510.29648343626909607No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG12240.2900845131841515No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12020.2848705758556782No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAGCCCATGAA10740.2545349404900153No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT10550.25003199461542475No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9420.22325131651917546No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC9190.2178003820394079No Hit
GGTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGT9150.2168523934342309No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8680.20571352732340156No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8590.20358055296175342No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8310.19694463272551463No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAAGTAGAGGACAAACGGGCAAC8180.19386366975868952No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT8050.19078270679186435No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAA8030.1903087124892759No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8010.1898347181866874No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7600.1801178349836235No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7600.1801178349836235No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7590.17988083783232925No Hit
GGATCATATTGTACAAAAGATCAGACACTGTTTTAGAAAACTTCCTGTTA7440.17632588056291562No Hit
GGCAACTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACT7310.1732449175960905No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT7300.17300792044479624No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7180.17016395462926534No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA6950.1647130201494978No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6920.1640020286956151No Hit
GTACTGTAGTGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6760.16021007427490722No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6690.1585510942158475No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATCAAACCTTATTATCCAGATC6680.1583140970645533No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC6520.15452214264384542No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6470.1533371568873742No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG6400.15167817682831453No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6380.15120418252572604No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT6310.14954520246666633No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6240.14788622240760665No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6230.1476492252563124No Hit
GTGTCTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC6200.14693823380242968No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA6170.14622724234854698No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6130.14527925374337No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6100.14456826228948727No Hit
TCTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGAT6040.1431462793817218No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6040.1431462793817218No Hit
GGATAATAAGGTTTGATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5960.14125030217136791No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT5880.13935432496101396No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5840.13840633635583702No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG5780.13698435344807156No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5730.13579936769160036No Hit
GGATTAAAGATAGGTACTGTAGTGGAATAAAGCCCAGTAAAGTTTCCCAC5720.13556237054030612No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5710.13532537338901185No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA5700.1350883762377176No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT5640.13366639332995217No Hit
CTCTACTTCCAGGATCAACAACAACCAGTACGGGACCATGCAAAACCTGC5570.1320074132708925No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5550.131533418968304No Hit
GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCC5500.1303484332118328No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5480.1298744389092443No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5460.12940044460665584No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5430.1286894531527731No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT5400.12797846169889038No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5400.12797846169889038No Hit
ATAGTCGCCTGATCGTTAAACCCATGTTAGTATTAACATAATTGACTACT5160.12229053006782858No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5150.12205353291653434No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5130.12157953861394585No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAAT5130.12157953861394585No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5100.12086854716006314No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5000.11849857564712073No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4990.11826157849582647No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4980.11802458134453223No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4960.11755058704194375No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4940.11707659273935528No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4920.11660259843676679No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG4910.11636560128547255No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4880.11565460983158982No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4880.11565460983158982No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4850.1149436183777071No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC4840.11470662122641286No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4790.11352163546994164No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGTC4780.11328463831864742No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC4760.11281064401605892No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTCTGATCTTTTGT4760.11281064401605892No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT4750.11257364686476469No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4740.11233664971347045No Hit
GTAGTGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4720.11186265541088196No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC4670.11067766965441077No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4640.10996667820052802No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4640.10996667820052802No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4620.10949268389793955No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4610.1092556867466453No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4600.10901868959535105No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG4600.10901868959535105No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA4590.10878169244405683No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATCAAAC4580.10854469529276257No Hit
GTTATGGTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGG4580.10854469529276257No Hit
ACATAATTGGAAGTTGGGGAACATTGCCACAGGATCATATTGTACAAAAG4570.10830769814146833No Hit
TTCTAAAACAGTGTCTGATCTTTTGTACAATATGATCCTGTGGCAATGTT4510.1068857152337029No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG4490.1064117209311144No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAA4480.10617472377982018No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAGCCC4460.10570072947723168No Hit
ATCTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGA4450.10546373232593743No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGAGCTTGGTGGAAGGC4420.10475274087205472No Hit
GCACTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACCCTCTGGGA4410.10451574372076049No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4390.10404174941817199No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4380.10380475226687774No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT4350.10309376081299503No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4280.10143478075393533No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA4250.10072378930005262No Hit
CAGTTGGACCCTGCATTCGGAGCCAACTCAAACAATCCAGATTGGGACTT4230.10024979499746413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)