FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454277
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC26720.5881873834686766No Hit
CCTCAAGCCTATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCGGA24060.5296328011323488No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC20320.4473041778474367No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG20200.4446626177420384No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19470.4285931271008658No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT16960.37334049489628574No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG15650.3445034637456882No Hit
GTTATATAGAATACCAGCCTTCCAGAGGGTATGTAAATAATGTCGTGTTT15430.3396606035524581No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15030.3308554032011306No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14840.32667293303425005No Hit
CTATATAACAGGGAAACCTCACGTAGCGCATCATTTTGCGGGTCACCATA14120.31082357240186054No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA13740.3024586320680994No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13330.29343330170798876No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG12830.28242680126882935No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT12590.2771436810580329No Hit
CTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTTG12210.26877874072427177No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12150.2674579606715726No Hit
CTCCTAGACACCGCCAAAGCTCTGTTTCAAGAAGCCTTAGAGTCTCCTGA11470.2524891200743159No Hit
ATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAA9810.21594753861630678No Hit
CTACGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG9600.21132480843185983No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT9480.20868324832646162No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG9360.2060416882210634No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC9220.20295986809809874No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCAAGCCTATAGACCACCAAATGCC9180.20207934806296596No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCTCCATTTACACAATGT8940.1967962278521695No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT8820.19415466774677126No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG8510.1873306374744924No Hit
ATGTAAGACCTTGGGCAAGATCTGATGGGCGTTCACGGTGGTCTCCATGC8460.1862299874305765No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8230.1811669972285632No Hit
AGCCTATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCGGAAACTA7930.17456309696506758No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7780.17126114683331978No Hit
GACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAACAGTG7700.16950010676305427No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7510.16531763659617368No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7200.1584936063238949No Hit
GGCTTGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA7180.15805334630632853No Hit
GTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG7180.15805334630632853No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC7140.15717282627119578No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAAAGGACGACAGAGT7090.1560721762272798No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6950.15299035610431522No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6930.15255009608674883No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6900.15188970606039928No Hit
CTATAGGCTTGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTC6860.15100918602526653No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6780.14924814595500102No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6650.14638645584081958No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATGCCGCCGTG6540.14396502574420453No Hit
ATATAACAGGGAAACCTCACGTAGCGCATCATTTTGCGGGTCACCATATT6520.14352476572663814No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6480.14264424569150538No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCCATCAG6470.1424241156827222No Hit
GTATTAACATAATCGACTACGAGATCCCTGGATGCTGGGTCTTGCAAATT6450.14198385566515584No Hit
TCCAGAGGGTATGTAAATAATGTCGTGTTTGGAAGTAATGATTAACTACC6430.14154359564758945No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6420.14132346563880627No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAACTTCAT6380.14044294560367354No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6360.14000268558610718No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG6330.1393422955597576No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCG6310.13890203554219122No Hit
TATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGA6210.13670073545435935No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT6180.13604034542800977No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6090.13405917534896108No Hit
TTATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6030.13273839529626197No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5840.1285559251293814No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG5740.12635462504154954No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5740.12635462504154954No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5730.12613449503276636No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5720.12591436502398318No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5690.1252539749976336No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGC5630.12393319494493449No Hit
GTATGTAAATAATGTCGTGTTTGGAAGTAATGATTAACTACCTGATCTGG5620.12371306493615128No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5610.1234929349273681No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5600.12327280491858492No Hit
GCTCATCGGAACTGACAACTCTGTCGTCCTTTCGCGGAAATATACATCGT5540.1219520248658858No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5530.1217318948571026No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT5510.12129163483953623No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5510.12129163483953623No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5480.12063124481318667No Hit
GTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAAGTTTAGGGAG5380.1184299447253548No Hit
CCCTCTGGAAGGCTGGTATTCTATATAACAGGGAAACCTCACGTAGCGCA5360.11798968470778841No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5340.11754942469022205No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAA5280.11622864463752292No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5280.11622864463752292No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5270.11600851462873973No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5240.11534812460239018No Hit
CCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACATTACCACAG5240.11534812460239018No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5230.11512799459360698No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGTAATGTTCCCCAACTTC5190.11424747455847423No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5190.11424747455847423No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACACGACATTATT5170.11380721454090786No Hit
CTGTCGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCCATCAGA5140.11314682451455829No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5060.11138578444429278No Hit
GCTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG5040.11094552442672642No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5020.11050526440916005No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC5000.11006500439159367No Hit
CCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCTTCTGGATT4990.10984487438281049No Hit
CATCAAAACCTCGCAAAGGCATGGGGACGAATCTTTCGGTTCCCAACCCT4980.1096247443740273No Hit
ATAAAGTGCTTCACGATGTTGTACAGACTTGGCCCCCAATACCACATCAT4970.10940461436524411No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4970.10940461436524411No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA4950.10896435434767775No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA4930.10852409433011136No Hit
CTGTTATATAGAATACCAGCCTTCCAGAGGGTATGTAAATAATGTCGTGT4910.108083834312545No Hit
AAATAATGTCGTGTTTGGAAGTAATGATTAACTACCTGATCTGGATAATA4890.1076435742949786No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4890.1076435742949786No Hit
ATTTACATACCCTCTGGAAGGCTGGTATTCTATATAACAGGGAAACCTCA4870.10720331427741224No Hit
GTATAAAGGGCTTCACGATGTTGTACAGACTTGGCCCCCAATACCACATC4860.10698318426862906No Hit
GTTTCGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG4850.10676305425984588No Hit
ATACTGCACTCAGGCAAGCCTTTCTCTGCTGGGGGGATGTAATGAATCTA4840.10654292425106267No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4840.10654292425106267No Hit
ATGCCGGGTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACC4830.10632279424227949No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4820.1061026642334963No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4810.10588253422471312No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4770.10500201418958037No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA4740.10434162416323081No Hit
GTACAATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCC4740.10434162416323081No Hit
ATCCAGATTGGGACTTCAACCCCATCAAGGACCACTGGCCAGCAGCCAAC4700.10346110412809806No Hit
GCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC4680.1030208441105317No Hit
ATATAGAATACCAGCCTTCCAGAGGGTATGTAAATAATGTCGTGTTTGGA4640.10214032407539894No Hit
GCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTT4630.10192019406661573No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG4620.10170006405783255No Hit
ATGTATATTTCCGCGAAAGGACGACAGAGTTGTCAGTTCCGATGAGCTTT4610.10147993404904937No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4600.10125980404026619No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT4600.10125980404026619No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4600.10125980404026619No Hit
GATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATT4590.10103967403148298No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4560.10037928400513343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)