FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454277
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC24990.5501048919491852No Hit
CCTCAAGCCTATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCGGA22910.5043178501222823No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG19910.438278847487326No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC19090.42022818676710466No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG16690.36739698465913967No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT16550.364315164536175No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16480.36277425447469275No Hit
GTTATATAGAATACCAGCCTTCCAGAGGGTATGTAAATAATGTCGTGTTT15370.33833982349975894No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA14000.30818201229646225No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG13950.30708136225254634No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12840.28264693127761253No Hit
CTCCTAGACACCGCCAAAGCTCTGTTTCAAGAAGCCTTAGAGTCTCCTGA12750.28066576119856385No Hit
CTATATAACAGGGAAACCTCACGTAGCGCATCATTTTGCGGGTCACCATA12750.28066576119856385No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT12150.2674579606715726No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG11890.26173458044320974No Hit
CTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTTG11830.26041380039051065No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT11720.2579923702938956No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG10810.23796053949462553No Hit
ATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAA9980.21968974876562097No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC9650.21242545847577576No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG9410.2071423382649793No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGTAATGTTCCCCAACTTC9050.19921765794878454No Hit
CTACGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG8520.18755076748327562No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCAAGCCTATAGACCACCAAATGCC8430.18556959740422693No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8390.1846890773690942No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCTCCATTTACACAATGT8210.1807267372109968No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT8150.17940595715829769No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT7980.1756637470089835No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG7440.16377672653469139No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCG7440.16377672653469139No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA7420.16333646651712502No Hit
ATGTAAGACCTTGGGCAAGATCTGATGGGCGTTCACGGTGGTCTCCATGC7310.16091503642050997No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7240.15937412635902765No Hit
AGCCTATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCGGAAACTA7240.15937412635902765No Hit
GGCTTGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA7180.15805334630632853No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6770.14902801594621784No Hit
GACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAACAGTG6770.14902801594621784No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6760.14880788593743466No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAAAGGACGACAGAGT6760.14880788593743466No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6750.14858775592865148No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6700.14748710588473551No Hit
GTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG6610.14550593580568683No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6510.14330463571785496No Hit
CTATAGGCTTGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTC6450.14198385566515584No Hit
GTATGTAAATAATGTCGTGTTTGGAAGTAATGATTAACTACCTGATCTGG6370.14022281559489036No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6340.1395624255685408No Hit
ATATAACAGGGAAACCTCACGTAGCGCATCATTTTGCGGGTCACCATATT6340.1395624255685408No Hit
CCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCTTCTGGATT6300.13868190553340803No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG6270.13802151550705846No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6260.13780138549827528No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6210.13670073545435935No Hit
TCCAGAGGGTATGTAAATAATGTCGTGTTTGGAAGTAATGATTAACTACC6190.13626047543679298No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6170.1358202154192266No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAACTTCAT6170.1358202154192266No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG6140.13515982539287702No Hit
GTATTAACATAATCGACTACGAGATCCCTGGATGCTGGGTCTTGCAAATT6100.1342793053577443No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCCATCAG6050.13317865531382833No Hit
TTATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6040.13295852530504515No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATGCCGCCGTG5960.13119748523477964No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5830.12833579512059823No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5820.12811566511181505No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5790.12745527508546547No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC5730.12613449503276636No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5720.12591436502398318No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5670.12481371498006724No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAA5650.12437345496250085No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5640.12415332495371767No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG5520.12151176484831941No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5480.12063124481318667No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5480.12063124481318667No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5460.1201909847956203No Hit
TATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGA5450.1199708547868371No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5440.11975072477805392No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5410.11909033475170437No Hit
GTTTCGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG5410.11909033475170437No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5390.11865007473413798No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5380.1184299447253548No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5340.11754942469022205No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5290.11644877464630611No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA5190.11424747455847423No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5180.11402734454969105No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC5160.11358708453212468No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT5140.11314682451455829No Hit
CCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACATTACCACAG5140.11314682451455829No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5120.11270656449699193No Hit
CCCTCTGGAAGGCTGGTATTCTATATAACAGGGAAACCTCACGTAGCGCA5100.11226630447942554No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5090.11204617447064236No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACACGACATTATT5090.11204617447064236No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5080.11182604446185918No Hit
CTGTAGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCCATCAGA5080.11182604446185918No Hit
CTGTCGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCCATCAGA5030.11072539441794324No Hit
AAATAATGTCGTGTTTGGAAGTAATGATTAACTACCTGATCTGGATAATA4980.1096247443740273No Hit
GTGGTAATGTTCCCCAACTTCCAATTATGTAACCCATGAAGTTTAGGGAG4960.10918448435646093No Hit
CATCAAAACCTCGCAAAGGCATGGGGACGAATCTTTCGGTTCCCAACCCT4960.10918448435646093No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGC4950.10896435434767775No Hit
CTGTTATATAGAATACCAGCCTTCCAGAGGGTATGTAAATAATGTCGTGT4950.10896435434767775No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC4930.10852409433011136No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG4900.10786370430376181No Hit
GCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC4850.10676305425984588No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4850.10676305425984588No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4830.10632279424227949No Hit
ATACTGCACTCAGGCAAGCCTTTCTCTGCTGGGGGGATGTAATGAATCTA4790.10544227420714673No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC4740.10434162416323081No Hit
GTGAAGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATA4660.1025805840929653No Hit
GTACAATATGATCCTGTGGTAATGTTCCCCAACTTCCAATTATGTAACCC4640.10214032407539894No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT4630.10192019406661573No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4630.10192019406661573No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA4620.10170006405783255No Hit
TCACCATACTGCACTCAGGCAAGCCTTTCTCTGCTGGGGGGATGTAATGA4620.10170006405783255No Hit
CTCCTCAAGCCTATAGACCACCAAATGCCCCTATCTTATCAACTCTTCCG4610.10147993404904937No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGC4590.10103967403148298No Hit
GTATAAAGGGCTTCACGATGTTGTACAGACTTGGCCCCCAATACCACATC4590.10103967403148298No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4580.1008195440226998No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4580.1008195440226998No Hit
ACATTATTTACATACCCTCTGGAAGGCTGGTATTCTATATAACAGGGAAA4560.10037928400513343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)