FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224042
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACAAGGTGGGAGTGGGAGCATTCGGGCCAGGGTTCACCCCTCCCCAT17380.7757474045045125No Hit
CCTGGAAGGTGGGAGTGGGAGCATTCGGGCCAGGGTTCACCCCTCCCCAT11620.5186527526088858No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10620.4740182644325618No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10330.4610742628614278No Hit
CCTTGTTGGCGTCCGGCCAATTGTCCTTGTGTGGATTGAGGTCCCAATCT8980.40081770382339027No Hit
GGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG8890.3968005998875211No Hit
TAAACGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATC8330.3718052865087796No Hit
GGTTTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG8050.35930762981940884No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7430.33163424715008794No Hit
CCTTCCAGGCGTCCGGCCAATTGTCCTTGTGTGGATTGAGGTCCCAATCT7400.3302952125047982No Hit
CCTTCCAGGCGTCGGGCCAATTGTCCTTGTGTGGATTGAGGTCCCAATCT7010.3128877621160318No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGACCT7010.3128877621160318No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6980.3115487274707421No Hit
ATAAACGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCAT6970.3111023825889789No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAAAGTCCAA6960.3106560377072156No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6800.3035145195990038No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6620.2954803117272654No Hit
ATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC6460.28833879361905357No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6290.2807509306290785No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA5610.2503994786691781No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5590.2495067889056516No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5570.24861409914212515No Hit
GGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT5510.2459360298515457No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5120.2285285794627793No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4940.22049437159104096No Hit
GTGTCCCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGCTGCTGG4720.21067478419224966No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT4620.20621133537461725No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA4380.1954990582122995No Hit
GAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATGGGGAG4160.18567947081350816No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGAGTAAAA3880.17318181412413744No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG3740.16693298577945206No Hit
GGCAAAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAA3680.16425491648887264No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTTCATCAAACTCTTCAAG3670.16380857160710938No Hit
GCCTAAGGGCTCCACCCCAACAGGCCCCCATGGGGAGGGGTGAACCCTGG3650.1629158818435829No Hit
CATGTATACAAGCTAAACAAGCTTTTACTTTCTCGCCAACTTACAAGGCC3580.15979146767124022No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3550.1584524330259505No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3390.15131091491773865No Hit
GGGCACACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATC3380.1508645700359754No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAAAACCCGTG3380.1508645700359754No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGC3370.15041822515421216No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT3370.15041822515421216No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3370.15041822515421216No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAAACTCTATG3310.1477401558636327No Hit
GCATATAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC3270.14595477633657974No Hit
TCCTAACATTCATTTGCAGGAAGACATTGTTGATAGGTGCAAGCAATTTG3250.14506208657305328No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT3240.14461574169129002No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3220.14372305192776355No Hit
TCCTCTGCCGATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCA3180.14193767240071056No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTC3160.1410449826371841No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATGAGAGGACAAACGGGCAAC3110.1388132582283679No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT3100.13836691334660464No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA2980.13301077476544576No Hit
GGAACATATTGTGCAAAAAATCAAATTATGTTTTAGAAAACTTCCTGTAA2970.13256442988368253No Hit
CTCTCATTCCAGGATCATCAACCACCAGCACCGGACCATGCAGAACCTGC2970.13256442988368253No Hit
CCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGCAGCACCTCCTCCTG2930.13077905035662954No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG2920.13033270547486633No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT2860.12765463618428688No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT2860.12765463618428688No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2860.12765463618428688No Hit
GCTCAGAATACTGCCTCTGCCATATCATCAACCTTCTCGAAGACTGGGGA2860.12765463618428688No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGACCT2840.12676194642076039No Hit
ATGTCAGGAAAAGAAGGAGTTTGCCAATCAGGATTAAAGTTAGGTACCGT2780.12408387713018094No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGATGATATGGCAGAGGCAGTA2780.12408387713018094No Hit
GAGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACC2700.12051311807607502No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2670.1191740834307853No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG2630.11738870390373232No Hit
CGCCTGGAAGGTGGGAGTGGGAGCATTCGGGCCAGGGTTCACCCCTCCCC2620.1169423590219691No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG2600.11604966925844262No Hit
GCATAAACGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC2590.11560332437667938No Hit
CCTTTAACCTGATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG2580.11515697949491613No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG2570.1147106346131529No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA2570.1147106346131529No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC2510.11203256532257345No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2490.11113987555904697No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA2470.11024718579552048No Hit
GGCCCGACGCCTGGAAGGTGGGAGTGGGAGCATTCGGGCCAGGGTTCACC2450.109354496031994No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA2440.10890815115023075No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC2420.10801546138670427No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA2400.10712277162317779No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC2380.10623008185965133No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA2370.10578373697788808No Hit
CTATTCGAGATCTTCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTA2360.10533739209612483No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG2320.10355201256907187No Hit
CTATTCGAGATCTTCTCGACTCCGCCTCTGCTCTGTATCGGGAGGCCTTA2300.1026593228055454No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA2300.1026593228055454No Hit
AGTTTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG2290.10221297792378214No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATAATTTGATTTTTTGC2260.10087394327849243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)