FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509402
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAACCAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC13920.27326158907895925No Hit
CTGTTCACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT12950.25421965363308346No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12950.25421965363308346No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12680.24891932108629333No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT11580.22732537367344455No Hit
CTTTAGGACATGGACATTGACGCCTATAAAGAATTTGGAGCTTCTGTGGA11030.21652839996702017No Hit
CGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTCGCTTG10610.2082834382275688No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10450.2051425004220635No Hit
GGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA9960.1955233783927036No Hit
TATTCACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTCC9750.19140089752297793No Hit
GATGCTATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT9590.18825995971747264No Hit
CTTTTATATAAGATCCCCGCTTTCCAAAGAGTGTGTAAATAATGTCGCGT9520.1868857994275641No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAA9280.18217439271930616No Hit
TACCCATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAA8860.1739294309798548No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8670.17019956733581731No Hit
ATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCG8530.16745124675600018No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA8420.1652918520147153No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG8410.16509554340187121No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8030.15763581611379618No Hit
CCTATCCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA8020.1574395075009521No Hit
CCTCCGGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7870.1544948783082909No Hit
CCTCAGGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7840.15390595246975866No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7780.15272810079269417No Hit
CCTTTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTCACATCTATCAA7660.15037239743856523No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7630.14978347160003297No Hit
GTTGTTGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGATAGTC7550.14821300269728035No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA7420.1456609907303073No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7350.14428683044039875No Hit
GCAAACGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACA7220.1417348184734257No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT7220.1417348184734257No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT7110.13957542373214082No Hit
GATGAGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT6910.13564925147525922No Hit
ACGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6770.1329009308954421No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6630.130152610315625No Hit
GGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG6460.12681536389727563No Hit
GAACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6240.12249657441470588No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCTAGGAACCTCTATG6210.12190764857617363No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6120.1201408710605769No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6070.1191593279963565No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCA6060.11896301938351243No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6050.11876671077066835No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6000.11778516770644795No Hit
CCTGCCGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC5930.1164110074165394No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGACCATGCAGTGGAA5870.11523315573947492No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5820.11425161267525452No Hit
ATCATCAACAACCAGCACCGGACCATGCAAAACCTGCACAGCTCCTGCTC5800.11385899544956635No Hit
ATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTGGAC5770.11327006961103411No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5690.11169960070828147No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG5590.10973651457984067No Hit
ATACAGAGCAGTAGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT5490.10777342845139988No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5460.10718450261286763No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAAAGTCCAA5430.10659557677433541No Hit
CTCCTGAACATTGTTCACATCACCATACGGCACTCAGGCAAGCTATTCTG5400.10600665093580317No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5370.1054177250972709No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5330.10463249064589461No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5130.100706318389013No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5100.10011739255048074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)