FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509402
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12920.25363072779455126No Hit
ACGTAACCAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC12370.24283375408812685No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12280.24106697657253015No Hit
CTTTAGGACATGGACATTGACGCCTATAAAGAATTTGGAGCTTCTGTGGA11790.23144785454317024No Hit
CTGTTCACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT11010.21613578274133197No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10810.21220961048445042No Hit
CCTGCCGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC10790.21181699325876222No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT10740.2108354501945418No Hit
CTTTTATATAAGATCCCCGCTTTCCAAAGAGTGTGTAAATAATGTCGCGT10680.20965759851747737No Hit
GGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA10650.2090686726789451No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAA9500.18649318220187594No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG9450.18551163913765553No Hit
TACCCATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAA9180.18021130659086537No Hit
TATTCACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTCC9100.17864083768811273No Hit
CCTCAGGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8900.17471466543123113No Hit
CGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTCGCTTG8810.1729478879156344No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8490.16666601230462386No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8450.16588077785324754No Hit
GATGCTATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT8250.16195460559636593No Hit
CCTCCGGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8050.15802843333948433No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT7970.1564579644367317No Hit
CCTTTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTCACATCTATCAA7840.15390595246975866No Hit
ATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCG7840.15390595246975866No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA7710.1513539405027856No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7600.14919454576150074No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7490.14703515102021586No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7440.14605360795599548No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7270.1427163615376461No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA7110.13957542373214082No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG7090.13918280650645265No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT6940.13623817731379145No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6690.13133046199268947No Hit
ACGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6590.12936737586424868No Hit
CCTATCCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA6490.12740428973580786No Hit
GGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG6460.12681536389727563No Hit
GATGAGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT6320.1240670433174585No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6270.12308550025323811No Hit
GTTGTTGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGATAGTC6230.12230026580186179No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6210.12190764857617363No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGACCATGCAGTGGAA6150.12072979689910915No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6150.12072979689910915No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6110.11994456244773283No Hit
GCAAACGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACA6110.11994456244773283No Hit
ATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTGGAC6100.11974825383488875No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6050.11876671077066835No Hit
GAACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6030.1183740935449802No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCA5930.1164110074165394No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCTAGGAACCTCTATG5760.11307376099819004No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGG5350.10502510787158276No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5270.10345463896883013No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5150.10109893561470115No Hit
CTCCTGAACATTGTTCACATCACCATACGGCACTCAGGCAAGCTATTCTG5140.10090262700185708No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5130.100706318389013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)