FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491453
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32020.651537379973263No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT14340.2917878210123857No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA13360.27184695179396606No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13130.2671669518753574No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12530.2549582564355086No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11670.23745912630505867No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11490.23379651767310405No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10240.20836173550675244No Hit
ATATAAAAGAGAGTCCACACGTAGCGCTTCATTTTGCGGGTCACCATATT8570.17438086653250667No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT8120.1652243449526201No Hit
GTATTATCCAGAGCATGTAGTTGATCATTACTTCCAGACGCGACATTATT8110.16502086669528926No Hit
CTATATGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTC8030.16339304063664278No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7790.15850956246070327No Hit
CGTTAATATGGGCCTAAAACTTAGACAATTATTGTGGTTTCACATTTCCT7710.15688173640205674No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTTCAAG7690.15647477988739514No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCACG7610.15484695382874863No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGG7080.14406260619021555No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7010.14263825838889985No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA6950.14141738884491498No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT6820.1387721714996144No Hit
CCTCAGGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6510.1324643455223592No Hit
CTCCAAAAGACACCAAATATTCAAGAACAACGCTTCTCCCAAAAGTAAGA6500.1322608672650284No Hit
ATCCAGCATCCAGGGAAGCAGTAGTCGGCTATGTCAACGTTAATATGGGC6370.12961564991972782No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6350.1292086934050662No Hit
GGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA6230.12676695431709645No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6180.12574956303044238No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6110.12432521522912669No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTGGATCGTC6110.12432521522912669No Hit
ATCCAGAGCATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACAC5990.12188347614115694No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5900.1200521718251796No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCT5890.11984869356784882No Hit
TGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5880.119645215310518No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5830.11862782402386393No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5810.1182208675092023No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5780.11761043273720986No Hit
AATATTTGGTATCCTTGGGATAAAACCTAGCTGGCATAATTAATTTTAGT5720.11638956319322499No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5710.11618608493589418No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCTACTTACAAGGCC5610.11415130236258604No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5600.11394782410525522No Hit
TCCCTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5520.11231999804660873No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACATGCTCTGGAT5470.11130260675995467No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTT5430.11048869373063142No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5410.11008173721596978No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5230.10641912858401516No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTAGGCGAGAAAGTAA5210.10601217206935352No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5190.1056052155546919No Hit
ATGCTGGATCGTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAAC5190.1056052155546919No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGT5160.10499478078269946No Hit
CTCCTAAGCTGTGCCTTAGGTGGCTTTGGGGCATGGACATTGACCCGTAT5140.10458782426803785No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5130.10438434601070704No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5120.10418086775337622No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTAGGCGA5100.10377391123871457No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5060.10295999820939133No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5020.10214608518006808No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACTT5010.10194260692273727No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTACACCTCACCATACGG4990.10153565040807566No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4960.1009252156360832No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4960.1009252156360832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)