FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491453
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31930.6497060756572857No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15780.32108869006802276No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA12710.25862086506746323No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12450.25333043037686204No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11850.24112173493701328No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11450.23298260464378076No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11220.22830260472517208No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10150.2065304311907751No Hit
ATATAAAAGAGAGTCCACACGTAGCGCTTCATTTTGCGGGTCACCATATT8310.16909043184190553No Hit
CTATATGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTC8170.16624173623927416No Hit
GTATTATCCAGAGCATGTAGTTGATCATTACTTCCAGACGCGACATTATT8160.16603825798194333No Hit
CGTTAATATGGGCCTAAAACTTAGACAATTATTGTGGTTTCACATTTCCT7720.15708521465938757No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCACG7710.15688173640205674No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA7650.1556608668580719No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTTCAAG7610.15484695382874863No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT7600.15464347557141783No Hit
CTCCAAAAGACACCAAATATTCAAGAACAACGCTTCTCCCAAAAGTAAGA7570.15403304079942537No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7460.15179477996878643No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCAGCTAGG7340.1493530408808167No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCT7110.14467304096220798No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT6960.14162086710224578No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6700.13633043241164464No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTGGATCGTC6570.1336852150663441No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACATGCTCTGGAT6390.13002260643438945No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6330.12880173689040458No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6320.12859825863307375No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6230.12676695431709645No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6050.12310434568514181No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTAGGCGAGAAAGTAA5890.11984869356784882No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5870.11944173705318718No Hit
CCTCAGGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5850.11903478053852555No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5830.11862782402386393No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTT5820.11842434576653312No Hit
TGCCTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5780.11761043273720986No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5750.11699999796521741No Hit
ATCCAGAGCATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACAC5690.11577912842123256No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5680.11557565016390174No Hit
ATCCAGCATCCAGGGAAGCAGTAGTCGGCTATGTCAACGTTAATATGGGC5670.11537217190657093No Hit
CTCCTAAGCTGTGCCTTAGGTGGCTTTGGGGCATGGACATTGACCCGTAT5530.11252347630393955No Hit
GGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5530.11252347630393955No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTAGGCGA5500.11191304153194709No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5410.11008173721596978No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5390.10967478070130818No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC5290.10763999812800004No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5280.10743651987066923No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGT5270.10723304161333841No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5240.10662260684134597No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCTACTTACAAGGCC5200.10580869381202271No Hit
AATATTTGGTATCCTTGGGATAAAACCTAGCTGGCATAATTAATTTTAGT5180.10540173729736109No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5100.10377391123871457No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5070.10316347646672215No Hit
TCCCTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5040.10255304169472972No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4990.10153565040807566No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4940.1005182591214216No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4930.10031478086409078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)