FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523873
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33900.6471034010151315No Hit
CTTTTATATAATATCCCTGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13960.26647679876611313No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13700.2615137638320738No Hit
ATATAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTCCATGC12380.23631681724387402No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG11890.2269634052527998No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11790.22505454566278468No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10670.20367531825461516No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10540.20119380078759547No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9980.19050418708351072No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9970.1903133011245092No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAGGTAAGA9680.1847776083134653No Hit
CATTTAAACCCTCACAAAACGAAAAGATGGGGATATTCCCTTAACTTCAT8920.17027027542935025No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8820.16836141583933512No Hit
GTCTTATATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8750.1670252141263245No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8640.16492546857730786No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA8250.15748091617624882No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAGTATT7820.14927281993918373No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7440.1420191534971262No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7400.14125560966112016No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA7370.1406829517841156No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7090.13533814493207325No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7070.1349563730140702No Hit
GCACCGGACCATGCAAAACCTGCACAACGTCTGCTCAAGGAACCTCTATG7050.13457460109606717No Hit
GTTGATAGATGTACGCAATTTGTGGGGCCCCTTACAATCAATGAAAACAG7000.1336201713010596No Hit
CTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAG6880.13132953979304143No Hit
GTCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC6690.1277027065720127No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6530.12464853122798845No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6370.12159435588396425No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6350.12121258396596121No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAA6270.11968549629394909No Hit
CTCCTACTCCCACCTTGTTGGCGTCCGGCCAGTTGTCCTTGTGCGGGTTG6260.1194946103349476No Hit
TACCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6210.11854018053994002No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6190.11815840862193701No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6150.11739486478593093No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6050.11548600519591581No Hit
TCCAGGGACTTAGTAGTCAGCTATGTCAACGTTAATATGGGCATAAAAAT6050.11548600519591581No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6010.11472246135990974No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5950.11357714560590065No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5810.11090474217987949No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5750.1097594264258704No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5620.10727790895885073No Hit
CTTTTATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5600.10689613704084769No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAACTCCCCCACTTT5590.10670525108184617No Hit
CTCATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACGGTACCTTGC5580.10651436512284465No Hit
ATTTAGTAGTATTGGGATAAAACCTAGCAGGCATAATTAATTTGAGTCTC5540.1057508212868386No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5510.10517816340983406No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5480.10460550553282953No Hit
TCTTCGGAGTTAATCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5430.10365107573782197No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5370.10250575998381287No Hit
AGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5310.10136044422980378No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5290.10097867231180077No Hit
CTCTTATATAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTTTC5260.10040601443479621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)