FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591912
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT29520.49872278311640916No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTATA28560.48250415602319263No Hit
ACTATATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA24230.4093513900714971No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23400.3953290353971536No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT21250.3590060684696374No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG16520.27909554122910163No Hit
TTATTATCCAGAGCATATAGTTAATCATTACTTCCAGACGAGACATTATT15200.25679492897592887No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13540.22875021962724187No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAGACACCCGTG13310.22486450688615875No Hit
ACTCACAACTGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13020.21996512995174958No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC12870.2174309694684345No Hit
AGTCTGTACAACACCTTGATTCCATTTATGCCGCTGTTACCCATTTTCTT12610.21303842463068837No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11730.19817134979523984No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT11530.1947924691508197No Hit
ATATGGAGGGGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA11490.19411669302193568No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT11370.19208936463528362No Hit
TTCAAGAACAGTGTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA11320.19124464447417858No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTATATGCTCTGGAT11250.19006203624863155No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA10840.1831353309275703No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTAAGA10810.1826284988309073No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10610.1792496181864872No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTGCGGGTCACCATATT10360.17502601738096205No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10290.173843409155415No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10130.1711403046398789No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC9950.1680993120599008No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG9550.16134155077106055No Hit
GTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAA9530.16100366270661853No Hit
GTATAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC9200.15542850964332536No Hit
GCATATAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCTTT9040.1527254051277893No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT8770.1481639162578221No Hit
ATGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTA8560.14461609158118097No Hit
GTACAACACCTTGATTCCATTTATGCCGCTGTTACCCATTTTCTTTTGTC8510.14377137142007596No Hit
GATGTATACTTGCGGGAAAGCACGACAGAATTGTCAGTCCCGATGAGTTT8400.1419129870656449No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATATAGTTAATCATTAC8300.14022354674343485No Hit
GATTAACTATATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGC8210.13870305045344578No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT8110.13701361013123572No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGTTGGATCATC8010.13532416980902567No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTATATGCTCTGG7950.13431050561569963No Hit
ATCCAACATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC7930.1339726175512576No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA7900.13346578545459462No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC7870.1329589533579316No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTATAATATGTT7840.13245212126126857No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7710.1302558488423955No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACATAGCGCC7590.1282285204557434No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAA7570.1278906323913014No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7490.12653908013353338No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7470.12620119206909133No Hit
CCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAACTCCACCACGTT7400.1250185838435443No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA7360.12434280771466029No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGATTCCATTTATGCC7340.12400491965021829No Hit
CTATATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATAT7230.1221465352957872No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7200.1216397031991242No Hit
GGAACATATTATACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7180.12130181513468218No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCA7160.12096392707024017No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6910.11674032626471503No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6910.11674032626471503No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC6880.11623349416805201No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT6820.11521982997472596No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6760.11420616578139994No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC6660.11251672545918988No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATATAG6500.10981362094365378No Hit
TCCTCAAGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG6480.10947573287921177No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAAAGCACGACAGAAT6460.10913784481476976No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTT6320.10677262836367568No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6310.10660368433145467No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA6310.10660368433145467No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6270.10592790820257066No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6220.10508318804146562No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAATTCAGACA6180.10440741191258161No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT6140.10373163578369757No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC6120.10339374771925557No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6100.10305585965481355No Hit
GTGCTGGTGGGAGTAGAAATGCTTCCTGGGAAGACACTGTTGGCAGGTGA6030.10187325142926651No Hit
ATCCAGAGCATATAGTTAATCATTACTTCCAGACGAGACATTATTTACAC6000.10136641933260349No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5970.10085958723594048No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACG5960.10069064320371948No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAT5940.10035275513927747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)