FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660401
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN106531.6131108220611416No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12150.18397912783293788No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11380.17231954524599447No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT11360.1720166989450349No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11270.17065389059071687No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10810.16368842566864678No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10130.15339165143602146No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGTCTAGTTT9500.14385199295579504No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAGTTATGCCTGCTAGG9450.14309487720339611No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGTAATGTCAACGACCGACCT9150.1385521826890026No Hit
GTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCT9090.13764364378612387No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8780.13294952612125058No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8700.13173814091741232No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAATTGCATGTTCAGG8170.12371271394198374No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8120.1229555981895848No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTGTATTC8080.12234990558766566No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7960.12053282778190827No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7780.11780721107327215No Hit
CTCCAAAAGACACCAAATACTCAAGAACGATTTCTCTTCCAAAAGTAAGA7460.11296167025791905No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7410.11220455450552014No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC7390.11190170820456057No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7180.1087218220444851No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6970.10554193588440963No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG6860.103876281229132No Hit
GGGTAAAGGTGCAGATATTGGTTACGCAGAAAGGCCTTGTAAGTTGGCGA6830.10342201177769264No Hit
GGTTAGGATAAAACCTAGCAGGCATAACTAATTTTAATCTCCTTTTTTCA6800.1029677423262533No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6760.10236204972433416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)