FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660401
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN184002.7861859688280304No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11530.17459089250319124No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10940.16565692662488396No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10320.15626869129513735No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT10130.15339165143602146No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGTAATGTCAACGACCGACCT10010.15157457363026403No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9200.13930929844140152No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9050.13703795118420473No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8970.1358265659803665No Hit
GTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCT8870.13431233447556865No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAGTTATGCCTGCTAGG8850.13400948817460906No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGTCTAGTTT8800.13325237242221016No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAATTGCATGTTCAGG8600.13022390941261447No Hit
CTCCAAAAGACACCAAATACTCAAGAACGATTTCTCTTCCAAAAGTAAGA8010.12128994353430718No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTGTATTC7870.1191700194275902No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7790.11795863422375194No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7490.1134159397093584No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7460.11296167025791905No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7330.11099316930168186No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6990.1058447821853692No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6720.10175635712241501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)