FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456690
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30320.6639076835490157No Hit
ACCTAATTTTAATCCTGATTGGCATACTCCTTCTTTTCCTGACATTCATT14370.31465545556066477No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12400.27151897348310666No Hit
GTATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAA11210.2454619107052924No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC10460.22903939214784647No Hit
GGATAATAGGGTTTGATTCCTTTATCTAATGGCAAGTATTTAGTAACATT9920.21721517878648533No Hit
AAATTGTGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTAT9280.20320129628413147No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTTCCAATTACATATCCCATGAA9190.20123059405723792No Hit
CCTCCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC8610.18853051303947974No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA8300.18174253870240206No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8250.18064770413190567No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC8150.1784580349909129No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT8060.17648733276401934No Hit
GTATTTAGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC8060.17648733276401934No Hit
GTGTAAAAGGGGCCGCAAACCCCAAAAGACCCACAATTCGTTGACACACT7760.16991832534104098No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7710.1688234907705446No Hit
ACTAAATACTTGCCATTAGATAAAGGAATCAAACCCTATTATCCAGAGCA7520.16466311940265826No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT7120.15590444283868707No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6990.15305787295539644No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6930.15174407147080077No Hit
GTATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAA6840.14977336924390725No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTTTCCATGC6760.148021633931113No Hit
AGTCTAGACTCTGTGGTATTATGAGGATTTTTGTCAACAAGAAAAACCCC6750.14780266701701375No Hit
GTTGTGAGCATGCCCAGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG6590.14429919639142524No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6570.1438612625632267No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6560.14364229564912742No Hit
GCTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6530.14298539490682957No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6470.1416715934222339No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6400.14013882502353894No Hit
CTACCAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG6360.1392629573671418No Hit
ACCACAATTTAATCCTGATTGGCATACTCCTTCTTTTCCTGACATTCATT6190.13554051982745408No Hit
CTCCAAAAGACACCAAGTATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6130.1342267183428584No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCACCAACTTACAAGGCC6020.1318180822877663No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5780.1265628763493836No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA5770.12634390943528434No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT5620.12305940572379513No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5590.1224025049814973No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5520.12086973658280234No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5370.11758523287131314No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT5360.11736626595721386No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA5260.11517659681622107No Hit
CTCCTACTCCCACCTTGTTGGCATCCGGCCAGTTGTCCTTGTGTGGATTG5210.11408176224572467No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5200.1138627953316254No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5190.1136438284175261No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC5170.11320589458932756No Hit
GATTTAGATCTTCTGCGACGCGGTGATTGAGACCTTCGTCGGCGAGGCGA5080.11123519236243404No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG5060.11079725853423547No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGGATAATATGTT4980.10904552322144123No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4930.10795068865094484No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4900.107293787908647No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4890.10707482099454772No Hit
ATTTAATCCTGATTGGCATACTCCTTCTTTTCCTGACATTCATTTGCAGG4850.1061989533381506No Hit
GGAACATATTATCCAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA4840.10597998642405133No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4820.10554205259585277No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC4770.10444721802535636No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG4760.1042282511112571No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4750.1040092841971578No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4730.10357135036895926No Hit
ATCTAATGGCAAGTATTTAGTAACATTGGGATAAAATCTAGCAGGCATAA4620.10116271431386717No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4590.10050581357156933No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4590.10050581357156933No Hit
GTCCAACTGATGGTCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT4570.10006787974337077No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4570.10006787974337077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)