FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456690
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30130.6597473121811294No Hit
ACCTAATTTTAATCCTGATTGGCATACTCCTTCTTTTCCTGACATTCATT12230.26779653594341896No Hit
GTATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAA11350.24852744750268235No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11220.24568087761939172No Hit
GGATAATAGGGTTTGATTCCTTTATCTAATGGCAAGTATTTAGTAACATT10060.22028071558387527No Hit
CCTCCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC9530.20867546913661347No Hit
GTATTTAGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC9450.2069237338238192No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC9160.20057369331494013No Hit
AAATTGTGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTAT8650.18940638069587684No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC8640.18918741378177759No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT8160.17867700190501215No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTTCCAATTACATATCCCATGAA8010.17539249819352296No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7880.17254592831023233No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7620.16685278854365104No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7620.16685278854365104No Hit
GTGTAAAAGGGGCCGCAAACCCCAAAAGACCCACAATTCGTTGACACACT7560.16553898705905537No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT7470.16356828483216185No Hit
ACTAAATACTTGCCATTAGATAAAGGAATCAAACCCTATTATCCAGAGCA7300.1598458472924741No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7180.15721824432328274No Hit
AGTCTAGACTCTGTGGTATTATGAGGATTTTTGTCAACAAGAAAAACCCC6910.1513061376426022No Hit
GTATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAGTATTCAA6870.15043026998620507No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG6800.1488975015875101No Hit
GTTGTGAGCATGCCCAGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG6650.14561299787602094No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6560.14364229564912742No Hit
CTCCAAAAGACACCAAGTATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6490.14210952725043247No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA6180.1353215529133548No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6100.13356981760056055No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA6030.1320370492018656No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6000.13138014845956777No Hit
CTACCAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG6000.13138014845956777No Hit
GCTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5910.12940944623267425No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5790.12678184326348288No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5570.12196457115329874No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT5510.12065076966870307No Hit
ACCACAATTTAATCCTGATTGGCATACTCCTTCTTTTCCTGACATTCATT5490.1202128358405045No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5450.11933696818410737No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCACCAACTTACAAGGCC5450.11933696818410737No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5440.1191180012700081No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTTTCCATGC5330.11670936521491604No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT5290.1158334975585189No Hit
CTCCTACTCCCACCTTGTTGGCATCCGGCCAGTTGTCCTTGTGTGGATTG5240.1147386629880225No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5240.1147386629880225No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC5210.11408176224572467No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA5210.11408176224572467No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5130.11233002693293043No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG5120.11211106001883116No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4920.10773172173684556No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4910.10751275482274629No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4890.10707482099454772No Hit
GGAACATATTATCCAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA4860.10641792025224989No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAAT4830.10576101950995204No Hit
ATCTAATGGCAAGTATTTAGTAACATTGGGATAAAATCTAGCAGGCATAA4790.10488515185355493No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4720.10335238345485996No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4700.10291444962666141No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4650.101819615056165No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4570.10006787974337077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)