FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420424
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16750.398407322131943No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12800.30445455064411164No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10410.24760717751603142No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTT10320.24546648145681504No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA10130.24094723422069148No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9970.2371415523376401No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9270.22049169409929023No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG8580.2040796909786311No Hit
TTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACC8320.19789545791867258No Hit
GTGCTGGTGCGTGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAG8320.19789545791867258No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8080.19218693509409548No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT7800.18552699179875554No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7440.1769642075618899No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7350.1748235115026735No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6990.16626072726580787No Hit
CCTATTCTCCTCCCCTAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6750.16055220444123078No Hit
TCCACAGAGTATGCAAATAATGCCTAGTTTTGAAGTAATGATCAACTGCA6360.151275854851293No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6290.14961086902745802No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG6210.1477080280859323No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6180.14699446273286015No Hit
CCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAG6170.14675660761516945No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6090.14485376667364375No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGT6090.14485376667364375No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6060.14414020132057162No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5890.1400966643198295No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5800.1379559682606131No Hit
ATGATCAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG5770.13724240290754094No Hit
TTTTTTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT5670.13486385173063384No Hit
GTATGCAAATAATGCCTAGTTTTGAAGTAATGATCAACTGCATGTTCAGG5620.13367457614218028No Hit
GATCAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5480.1303446044945103No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5430.12915532890605674No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5410.12867961867067532No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT5390.1282039084352939No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTTAT5370.12772819819991246No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG5300.12606321237607748No Hit
CTTTAGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCC5210.12392251631686109No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5150.1224953856107168No Hit
GTGCTGGTGGAGCCTGGCATTGCCCCTTACCTACCATGCCATTCCTGACC5140.12225753049302608No Hit
CCCCTAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA5090.12106825490457254No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAAGTT5050.12011683443380967No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAATTAAGGGAG5010.11916541396304683No Hit
GAGTATGCAAATAATGCCTAGTTTTGAAGTAATGATCAACTGCATGTTCA4970.118213993492284No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA4950.11773828325690255No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4810.11440831160923258No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4800.11417045649154188No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4800.11417045649154188No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4760.11321903602077901No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4740.1127433257853976No Hit
AATGATCAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAG4730.11250547066770687No Hit
TCCCGATACAGAGCAGAGGCGGTATCGAGGAGATCTCGAATAGAAGGAAA4660.11084048484387189No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4640.11036477460849047No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4620.10988906437310905No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4600.10941335413772763No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4530.10774836831389265No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATA4480.10655909272543908No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGACGTTCACGGTGGTCTCCATGC4470.10632123760774836No Hit
TGATCAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGG4430.10536981713698552No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4340.10322912107776912No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTAAGTTTGAGTACAATATGTT4320.10275341084238769No Hit
ATCCTAACTTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4300.10227770060700625No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4270.10156413525393414No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4270.10156413525393414No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4260.10132628013624341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)