FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420424
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13400.3187258577055544No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10000.23785511769071221No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9890.2352387113961144No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9860.23452514604304225No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTT8380.19932258862481686No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8320.19789545791867258No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA7680.182672730386467No Hit
GTGCTGGTGCGTGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAG7210.1714935398550035No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7210.1714935398550035No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7030.1672121477365707No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT6930.16483359655966356No Hit
TTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACC6870.1634064658535193No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6800.1617414800296843No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGT6060.14414020132057162No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6000.14271307061442734No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5360.12749034308222176No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5310.12630106749376818No Hit
GTATGCAAATAATGCCTAGTTTTGAAGTAATGATCAACTGCATGTTCAGG5300.12606321237607748No Hit
CCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAG5200.12368466119917036No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5110.12154396513995396No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4850.11535973207999545No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG4820.11464616672692331No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4780.11369474625616044No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4750.11298118090308831No Hit
TTTTTTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT4690.11155405019694403No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG4650.11060262972618119No Hit
CCTATTCTCCTCCCCTAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA4640.11036477460849047No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAAGTT4570.10869978878465548No Hit
GTGCTGGTGGAGCCTGGCATTGCCCCTTACCTACCATGCCATTCCTGACC4550.10822407854927407No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTTAT4370.10394268643084123No Hit
GATCAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4360.10370483131315053No Hit
ATGATCAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4280.10180199037162484No Hit
GAGTATGCAAATAATGCCTAGTTTTGAAGTAATGATCAACTGCATGTTCA4270.10156413525393414No Hit
CCCTTAATTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4260.10132628013624341No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4240.100850569900862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)