FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454936
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGCTCAGG19890.43720435401902685No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15380.33806953065925754No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG13750.3022403151212478No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC12680.278720523326358No Hit
GTGTCAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC12130.2666309107215081No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12060.2650922327536181No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAAT11630.25564035380800815No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTATGGGTCACCATA11070.24333093006488823No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTGAGGTT10970.24113281868218825No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCACG10420.22904320607733836No Hit
ATCCTAACCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10340.22728471697117839No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10110.2222290607909684No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9820.21585453778113847No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG9550.2099196370478485No Hit
GTTTTAAAGTAATGATTAACTGCATGCTCAGGATAATAAGGTTTAATGCC9310.20464416972936855No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT9190.20200643607012855No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9170.20156681379358854No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG9090.19980832468742857No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9000.1978300244429986No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTATGGGTCACCATATT8820.1938734239541386No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8760.19255455712451863No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8730.19189512370970863No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA8340.18332248931717868No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8180.1798055111048587No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA8150.1791460776900487No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC8120.17848664427523872No Hit
GATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA8080.17760739972215872No Hit
GGATCATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA8030.17650834403080873No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7560.1661772205321188No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7460.16397910914941882No Hit
GTTGACATCTGTTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAG7300.16046213093709885No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGTGAGA7090.1558460970334289No Hit
CCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7040.1547470413420789No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC6940.1525489299593789No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6900.1516696854062989No Hit
GATTAACTGCATGCTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6810.14969138516186892No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA6800.14947157402359892No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6790.1492517628853289No Hit
CGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAA6720.14771308491743892No Hit
TTATTATCCTGAGCATGCAGTTAATCATTACTTTAAAACTAGGCATTATT6550.14397629556684896No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6520.14331686215203898No Hit
CATTTGAATCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6240.137162150280479No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6240.137162150280479No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6170.13562347231258903No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTAATAATGTCCTCCTGT6160.135403661174319No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCCTACAGACCACCAAATGCC6130.13474422775950903No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC6110.13430460548296905No Hit
TTATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6110.13430460548296905No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6090.13386498320642903No Hit
ATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5970.13122724954718903No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5930.13034800499410906No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA5920.13012819385583907No Hit
GTTCACATCTGTTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAG5800.12749046019659907No Hit
GTTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTGCC5740.12617159336697908No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG5680.12485272653735911No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5670.1246329153990891No Hit
ATATTATACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA5650.1241932931225491No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5650.1241932931225491No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5650.1241932931225491No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGCTCA5590.1228744262929291No Hit
GTTCTATCCTAACCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5510.12111593718676912No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5500.12089612604849914No Hit
GCTTTAGGGCATGGACATTGACCCCTATAAAGAATTTGGAGCTTCTGCGG5420.11913763694233914No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5400.11869801466579916No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5340.11737914783617916No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5300.11649990328309916No Hit
GGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC5270.11584046986828918No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT5190.11408198076212918No Hit
GATTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGAGGTAAAA5190.11408198076212918No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTG5170.11364235848558919No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5040.11078481368807921No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5030.11056500254980921No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4980.10946594685845921No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA4960.10902632458191922No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTA4950.10880651344364921No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA4900.10770745775229923No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATGAGGATTCAAATGTATA4890.10748764661402922No Hit
CATTTAAATCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4880.10726783547575923No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4860.10682821319921924No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4860.10682821319921924No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4850.10660840206094924No Hit
GATTAAGATTAATTATGCCTGCTAGGTTCTATCCTAACCTCACCAAATAT4840.10638859092267923No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTGTTACCAATTTTCTTTTGTC4820.10594896864613923No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT4770.10484991295478924No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAG4760.10463010181651926No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4730.10397066840170927No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTATAATA4730.10397066840170927No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4700.10331123498689926No Hit
CTTTACCACAGGATCATATTATACAAAAAATCAAGCAATGTTTTCGAAAA4620.10155274588073927No Hit
GTCCAAGGGATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGAC4600.10111312360419927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)