FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533313
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20270.38007699043526033No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11820.2216334497752727No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9340.1751316768951816No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8880.16650634805451958No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC6890.1291924254612207No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6830.12806738256896044No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC6690.12544228248701983No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6310.11831701083603813No Hit
TCTCTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6210.11644193934893768No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6000.11250428922602673No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5760.10800411765698567No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG5590.1048164961289149No Hit
TATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTG5550.10406646753407475No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5370.10069133885729394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)