FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533313
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20150.3778269046507398No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11500.21563322101655125No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT8250.15469339768578677No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC7570.14194291157350375No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6420.12037958947184861No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5750.10781661050827562No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5710.10706658191343545No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5350.10031632455987384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)