FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401016
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14940.37255371356753847No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14930.37230434695872483No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC13430.3348993556366828No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11690.2915095657031141No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9970.24861850898717255No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9970.24861850898717255No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9910.2471223093342909No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9620.23989067767869612No Hit
CTCACTGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCT9000.22442994793225207No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8920.22243501506174318No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8910.22218564845292954No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8730.21769704949428453No Hit
ATTGACAACAGTGCCAGCGGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7750.1932591218305504No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7570.18877052287190535No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7350.18328445747800587No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7130.17779839208410636No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6680.16657689468749376No Hit
GACCTGCTGCGAGCAAAACACGCTGCTAGGAGTTCCGCAGTATGGATCGG6640.1655794282522393No Hit
CAATATCTCTTCCAAAAATAATACAGGAAAGGTGAAACCACAATAGTTGT6470.1613401959024079No Hit
CTTTATACCTCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTGAA6250.15585413050850838No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6140.15311109781155863No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA6120.1526123645939314No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA6120.1526123645939314No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6070.15136553154986335No Hit
CCTTTGGAGTGTGGATTCGCACTCCTAGCGCTTACAGACCACCAAATGCC6010.14986933189698168No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5730.1428870668502005No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5700.14213896702375964No Hit
GGAAAGGTGAAACCACAATAGTTGTCTGAGTTTCAGGCCCATATTAACAT5610.13989466754443713No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5570.13889720110918266No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5570.13889720110918266No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5540.13814910128274183No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5490.13690226823867377No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5450.13590480180341932No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5450.13590480180341932No Hit
GATTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG5420.13515670197697846No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5390.13440860215053765No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGTTAG5360.1336605023240968No Hit
CTGCTGAGTTACTCTCTTTTTTGCCTAATGACTTCTTTCCTTCCATTCGA5350.13341113571528318No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5330.13291240249765596No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5290.13191493606240148No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5180.12917190336545176No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG5120.12767570371257006No Hit
CTGTAAGAGGGCCCACAAATTTTTGACATCTATCAATAATGTCCTCCTGT5100.12717697049494286No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTCATAAAACCAAACGTTGGG5070.126428870668502No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5040.12568077084206117No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5020.12518203762443395No Hit
AACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGAT5000.12468330440680671No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4870.12144153849222973No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4850.12094280527460251No Hit
ATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGAC4830.1204440720569753No Hit
CTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTA4700.1172023061423983No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4690.11695293953358471No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4600.11470864005426216No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4580.11420990683663494No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4580.11420990683663494No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCC4450.11096814092205796No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4440.11071877431324435No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4430.11046940770443074No Hit
CAGACAGATTCGTCCCCATGCCTTGTCGAGGTTTGGAAGACCAACCTCCC4420.11022004109561712No Hit
CTCCTAGCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4360.10872384144273546No Hit
AATTTTTGACATCTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAA4330.10797574161629461No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4270.10647954196341294No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4190.10448460909290402No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4150.10348714265764956No Hit
CCACAATAGTTGTCTGAGTTTCAGGCCCATATTAACATTGACATAGCCGA4120.10273904283120874No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4070.10149220978714066No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4060.10124284317832705No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4040.10074410996069982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)