FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401016
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17030.4246713348095837No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12030.29998803040277694No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11990.2989905639675225No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10480.2613362060366669No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10200.25435394098988573No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10080.25136154168412234No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10060.2508628084664951No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9960.24836914237835894No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9610.2396413110698825No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8660.21595148323258923No Hit
CTCACTGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCT8380.20896921818580805No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7860.19600215452750014No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA7200.17954395834580167No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7060.17605282582241108No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6760.16857182755800268No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6640.1655794282522393No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA6500.16208829572884872No Hit
ATTGACAACAGTGCCAGCGGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6490.1618389291200351No Hit
CCTTTGGAGTGTGGATTCGCACTCCTAGCGCTTACAGACCACCAAATGCC6380.15909589642308536No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6340.1580984299878309No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6270.1563528637261356No Hit
GACCTGCTGCGAGCAAAACACGCTGCTAGGAGTTCCGCAGTATGGATCGG5730.1428870668502005No Hit
CTTTATACCTCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTGAA5710.14238833363257325No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5650.14089213397969158No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5640.14064276737087797No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5610.13989466754443713No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5490.13690226823867377No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5420.13515670197697846No Hit
CTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTA5380.13415923554172404No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5350.13341113571528318No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5340.13316176910646957No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5320.13266303588884235No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTCATAAAACCAAACGTTGGG5310.13241366928002873No Hit
CAATATCTCTTCCAAAAATAATACAGGAAAGGTGAAACCACAATAGTTGT5270.13141620284477426No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5150.1284238035390109No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5150.1284238035390109No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5020.12518203762443395No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5010.12493267101562032No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4990.12443393779799308No Hit
CTGTAAGAGGGCCCACAAATTTTTGACATCTATCAATAATGTCCTCCTGT4920.1226883715362978No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGTTAG4760.11869850579527999No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4740.11819977257765275No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4680.11670357292477108No Hit
CTCCTAGCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4670.11645420631595747No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4610.11495800666307579No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4580.11420990683663494No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4470.1114668741396852No Hit
GATTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4460.11121750753087159No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCACG4450.11096814092205796No Hit
GGAAAGGTGAAACCACAATAGTTGTCTGAGTTTCAGGCCCATATTAACAT4410.10997067448680353No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4380.10922257466036268No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4350.10847447483392184No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4340.10822510822510822No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4320.107726375007481No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4320.107726375007481No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4280.10672890857222654No Hit
CTGCTGAGTTACTCTCTTTTTTGCCTAATGACTTCTTTCCTTCCATTCGA4230.10548207552815848No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4220.10523270891934487No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4210.10498334231053125No Hit
ATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGAC4110.10248967622239512No Hit
ATAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4110.10248967622239512No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4080.10174157639595428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)