FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401937
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22920.5702386194851432No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16520.4110096855974942No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16500.4105120951790952No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGATAAAGGCATTAAACCTTAT13260.3299024473984729No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11520.2866120809977683No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9200.22889159246349552No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8470.21072954219193557No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8380.20849038530914046No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8210.20426086675274982No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG7410.18435725001679368No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCTTTGGATAAAGGCATTAAAC6880.17117110392922275No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6840.17017592309242494No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT6410.15947772909684851No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA6290.1564921865864551No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAAGGCAAATATTTGGTAAGGTT6270.1559945961680562No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG5870.14604278780007812No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG5860.14579399259087866No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5830.1450476069632803No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG5600.1393253171516929No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5590.13907652194249348No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5430.13509579859530224No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5410.13459820817690335No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5400.1343494129677039No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5320.13235905129410827No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC5240.13036868962051268No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5030.12514399022732417No Hit
GTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGTGTTTCTTCAAAA4720.11743133874214118No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG4700.11693374832374229No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4280.10648434953736531No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4190.10424519265457023No Hit
TCTTTACACTGCTCCTATAAATTGAATTATTATGTACTCTTTTGAATCTA4050.10076205972577791No Hit
CCCTAACAGTAACCCTAACACGAAAAACAAAAACAGAACTATTCACTCAC4050.10076205972577791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)