FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401937
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22710.5650139200919547No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18350.4565392088809938No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16040.39906751555592046No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGATAAAGGCATTAAACCTTAT11060.2751675013745935No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10050.25003918524544894No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10020.24929279961785053No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8970.22316930265190815No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA7910.1967970104767663No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7900.19654821526756683No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7850.19530423922156956No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG7180.1786349602052063No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7110.17689339374081015No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG6450.16047290993364632No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6440.16022411472444686No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6270.1559945961680562No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAAGGCAAATATTTGGTAAGGTT6020.1497747159380699No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5970.14853073989207266No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG5890.146540378218477No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5830.1450476069632803No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCTTTGGATAAAGGCATTAAAC5670.1410668836160891No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG5470.13609097943210005No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC5300.13186146087570938No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG4780.11892410999733789No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT4750.11817772436973953No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4710.11718254353294173No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4620.11494338665014665No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4370.10872350642016036No Hit
ATATGATACTAGAATGGTGGACAGCTGTCATTATGCATTTATCCAAACCC4260.1059867591189664No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4250.10573796390976695No Hit
TCTTTACACTGCTCCTATAAATTGAATTATTATGTACTCTTTTGAATCTA4060.10101085493497738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)