FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442458
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28040.6337324672624294No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG25570.5779079596255464No Hit
ATCCTAACCTTACCAAATATTTCCCATTGGATAAAGGCATTAAACCTTAT15510.3505417463352454No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14760.33359098490704203No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13200.298333401136379No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12500.2825126904700559No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11030.24928919807077735No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10780.24363894426137622No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10370.2343725280139584No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10290.23256444679495003No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10040.2269141929855489No Hit
GGATAATAAGGTTTAATGCCTTTATCCAATGGGAAATATTTGGTAAGGTT9910.22397606100466033No Hit
CTCCAAAAGACACCAAATACTCAATAACAGTTTCTCTTCCAAAAGTAAGA9560.21606570567149877No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9230.2086073706430893No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9220.20838136049071326No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9120.20612125896695277No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8710.19685484271953496No Hit
CATGTATACAGGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8670.19595080211003077No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8520.1925606498243901No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8430.1905265584530057No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8380.18939650769112548No Hit
GTTTTATCCTAACCTTACCAAATATTTCCCATTGGATAAAGGCATTAAAC8050.181938172662716No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC7870.1778699899199472No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7540.17041165489153773No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7410.16747352291064915No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7190.16250129955837617No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7100.16046720818699176No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6980.15775508635847924No Hit
GTTGTCAATATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG6970.15752907620610318No Hit
CCTTTATCCAATGGGAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG6850.15481695437759063No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6660.15052276148244578No Hit
CTATATAAGCGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6420.14509851782542071No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA6400.14464649752066863No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT6360.14374245691116444No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6300.1423863959969082No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA6240.14103033508265192No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6070.13718816249225915No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA6050.13673614218750707No Hit
AGTCTGTACAACATCGTGAGGCCCTTTATACCTCTATTACCAATTTTCTT6020.13605811173037893No Hit
CTCAATAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5970.1349280609684987No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5960.13470205081612266No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGT5890.13311997974949033No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGCGAGAAACTACA5890.13311997974949033No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5710.12905179700672154No Hit
GAATCTGGCCACCTGGGTGGGAGGTAATTTGGAAGACCCAGCATCCAGGG5580.12611366502583296No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5550.12543563456870482No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG5550.12543563456870482No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCCATTCG5520.12475760411157669No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5510.12453159395920065No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAATGGGAAATATTTGGTA5410.12227149243544021No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5380.12159346197831206No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5280.11933336045455163No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5280.11933336045455163No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA5240.11842931984504745No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC5220.11797729954029536No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5220.11797729954029536No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5210.1177512893879193No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5160.11662123862603908No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5150.11639522847366303No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAATGGG5040.11390911679752655No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4970.11232704573089422No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA4930.11142300512139004No Hit
GGGAATTAGTAGTCAACTATGTCAATGTTAATATGGGCCTAAAACTCAGA4850.1096149239023817No Hit
GTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTG4830.10916290359762962No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC4820.10893689344525356No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4800.10848487314050147No Hit
GTACAACATCGTGAGGCCCTTTATACCTCTATTACCAATTTTCTTTTGTC4770.10780684268337334No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4750.10735482237862126No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4700.10622477161674101No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4700.10622477161674101No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT4680.10577275131198893No Hit
CTCTAAGGCCTCCCGAAACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4680.10577275131198893No Hit
CAATAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAAT4660.10532073100723684No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCT4640.10486871070248475No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTTCACCAAGCTCTGCTAG4630.10464270055010871No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4550.10283461933110036No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4540.1026086091787243No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4510.10193057872159618No Hit
TATTAACATTGACATAGTTGACTACTAATTCCCTGGATGCTGGGTCTTCC4500.10170456856922012No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC4490.10147855841684408No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT4480.10125254826446803No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAAT4470.101026538112092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)