FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396734
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46561.1735822994752152No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG26360.6644250303729955No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15450.3894296934469947No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9880.24903335736286783No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9580.2414716157425378No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9380.23643045466231782No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8650.2180302167195148No Hit
TTATTAATAGATGTCAACAATTCGTGGGCCCTCTTACAGTCAATGAAAAA7950.20038615293874484No Hit
ATCCCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG7710.19433675964248084No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7420.18702707607616187No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7100.17896121834780987No Hit
CTCTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7090.17870916029379885No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6730.16963507034940287No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6630.1671144898092929No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6590.16610625759324887No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6520.1643418512151719No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6500.16383773510714988No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6500.16383773510714988No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6190.1560239354328089No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5760.1451854391103359No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5730.1444292649483029No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5380.13560723305791791No Hit
GATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT5370.1353551750039069No Hit
ATCCTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG5320.1340948847338519No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG5000.1260290270054999No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4890.1232563884113789No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4800.1209878659252799No Hit
TTATTGATAGATGTCAACAATTCGTGGGCCCTCTTACAGTCAATGAAAAA4730.1192234595472029No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4710.11871934343918092No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4630.1167028790070929No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA4520.11393024041297191No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4490.11317406625093891No Hit
TGGACAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTAC4420.11140965987286192No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4360.10989731154879591No Hit
CACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4270.10762878906269693No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4250.10712467295467493No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATA4160.10485615046857592No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4150.10460409241456492No Hit
GATTAAGATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT4080.10283968603648791No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC4040.10183145382044394No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4000.10082322160439992No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA3980.10031910549637793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)